高效液相色谱-质谱联用法同时测定大鼠血浆中蒿甲醚和氨芳碱的含量

IF 1 4区 工程技术 Q4 CHEMISTRY, ANALYTICAL
Ripal Shah,  Tejal Soni, Dinal Patel, Jenee Christian, Mansi Dholakia, Mehul Patel, Kishor Sorathia
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引用次数: 0

摘要

以卡马西平为内标,建立并优化了同时定量大鼠血浆中蒿甲醚(ART)和氨苯曲明(LUF)的LC-MS /MS方法。质谱仪在电喷雾电离(ESI)的正电离模式下工作,产生的产物离子在m/z 163.05(ART), m/z 511.95 (LUF)和m/z 194.1 (IS)。色谱分离采用Waters™X Select C18色谱柱(100 mm × 4.6 mm, 2.5µm),流动相为0.2%甲酸水溶液:甲醇(10:90),流速为0.5 mL/min。在6分钟的时间内,ART、LUF和IS的保留时间分别为4.30分钟、1.60分钟和2.46分钟。液液萃取(LLE)法以乙酸乙酯加酸化甲醇为萃取回收率最佳,ART和LUF的平均回收率分别为92.12%和91.07%。校正曲线在5 ~ 1000 ng/mL范围内呈线性,回归系数≥0.9992。该方法精密度高(%CV: 0.16 ~ 1.89),定量下限为98.24 ~ 106.42%(定量限:5 ng/mL)。该方法表现出最小的基质效应,稳定性测试证实了分析物在台式和冻融条件下的完整性。据我们所知,这是第一个经过验证的LC-MS/MS方法,用于同时定量大鼠血浆中ART和LUF的临床前药代动力学研究,为常规高通量分析提供了一种新颖、灵敏、经济的分析工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Simultaneous Quantification of Artemether and Lumefantrine in Rat Plasma Using a Validated LC-MS/MS Method

Simultaneous Quantification of Artemether and Lumefantrine in Rat Plasma Using a Validated LC-MS/MS Method

A robust and reproducible LC–MS/MS method was developed and optimized for the simultaneous quantification of artemether (ART) and lumefantrine (LUF) in rat plasma, employing carbamazepine as an internal standard (IS). The mass spectrometer operated in positive ionization mode with electrospray ionization (ESI), yielding product ions at m/z 163.05(ART), m/z 511.95 (LUF), and m/z 194.1 (IS). Chromatographic separation was achieved using a Waters™ X Select C18 column (100 mm × 4.6 mm, 2.5 µm) with a mobile phase of 0.2 % formic acid in water: methanol (10:90) at a flow rate of 0.5 mL/min. Retention times for ART, LUF, and IS were 4.30 min, 1.60 min, and 2.46 min, respectively, within a 6-min runtime. The liquid–liquid extraction (LLE) method utilized ethyl acetate with acidified methanol for optimal analyte recovery, achieving mean recoveries of 92.12 % for ART and 91.07% for LUF. Calibration curves were linear over the range of 5–1000 ng/mL with regression coefficients of ≥ 0.9992. The method demonstrated high precision (%CV: 0.16–1.89) and accuracy (98.24–106.42 %) at the lower limit of quantification (LLOQ: 5 ng/mL). The method exhibited minimal matrix effects, with stability tests confirming analyte integrity under benchtop and freeze–thaw conditions. To the best of our knowledge, this is the first validated LC-MS/MS method developed for the simultaneous quantification of ART and LUF in rat plasma for preclinical pharmacokinetic studies, providing a novel, sensitive, and cost-effective analytical tool for routine high-throughput analysis.

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来源期刊
Accreditation and Quality Assurance
Accreditation and Quality Assurance 工程技术-分析化学
CiteScore
1.80
自引率
22.20%
发文量
39
审稿时长
6-12 weeks
期刊介绍: Accreditation and Quality Assurance has established itself as the leading information and discussion forum for all aspects relevant to quality, transparency and reliability of measurement results in chemical and biological sciences. The journal serves the information needs of researchers, practitioners and decision makers dealing with quality assurance and quality management, including the development and application of metrological principles and concepts such as traceability or measurement uncertainty in the following fields: environment, nutrition, consumer protection, geology, metallurgy, pharmacy, forensics, clinical chemistry and laboratory medicine, and microbiology.
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