Fed-batch bioreactor modeling

This paper describes an open-source computational tool developed for the modeling and simulation of fed-batch bioreactors, particularly for processes employing Chinese Hamster Ovary (CHO) cells, which are integral to biopharmaceutical manufacturing. The software provides a platform for researchers and industry professionals to simulate bioreactor dynamics and investigate the impact of various operational parameters, such as nutrient supply rates, oxygen concentrations, and temperature, prior to physical experimentation. The tool enables users to generate predictions of critical variables including cell density, nutrient consumption, and product concentration profiles over time. These predictions are derived from a mathematical framework based on a system of ordinary differential equations solved using the Runge–Kutta method. A notable capability of the software is the import of experimental data and the application of the Nelder–Mead algorithm for parameter optimization, allowing for the calibration of the model against empirical findings, thereby enhancing its predictive accuracy. The software supports in silico experimentation, which can contribute to reducing the time, cost, and resources associated with optimizing bioreactor configurations and scaling up production processes. By providing a refined and adaptable framework, this instrument assists in improving the understanding of bioreactor dynamics, optimizing biopharmaceutical production methodologies, and correlating theoretical models with practical bioreactor operations. The software is available as an open-source project to promote its adoption and continued development within the scientific community.