Lee C. Miller , Hilary G. Close , Kalina C. Grabb , Christine L. Huffard , Fuyan Li , David M. Karl , Kenneth L. Smith , Edward F. DeLong , Claudia R. Benitez-Nelson , Jeffrey C. Drazen , Brian N. Popp
{"title":"从特定化合物稳定同位素和微生物群落分析推断的北太平洋深海平原表层颗粒有机质向深海平原的转化","authors":"Lee C. Miller , Hilary G. Close , Kalina C. Grabb , Christine L. Huffard , Fuyan Li , David M. Karl , Kenneth L. Smith , Edward F. DeLong , Claudia R. Benitez-Nelson , Jeffrey C. Drazen , Brian N. Popp","doi":"10.1016/j.dsr.2025.104597","DOIUrl":null,"url":null,"abstract":"<div><div>Particulate organic matter (POM) produced in surface waters undergoes extensive reworking and breakdown by microbial and metazoan communities as it sinks to the abyssal seafloor and serves as the base of benthic and pelagic food webs. Here, we examined how various size classes of POM in the oligotrophic North Pacific Subtropical Gyre (Station ALOHA) and in the eutrophic California Current System (Station M) undergo microbial alteration throughout the water column. Compound-specific stable isotope analysis showed that sampling method strongly impacts the type of POM quantified as export to the deep sea. Moored abyssal sediment traps captured material that matched the isotopic composition of surface POM, indicating they collected large, fast-sinking particles, in contrast to the more heavily reworked particles collected with <em>in situ</em> filtration at the same depths. Extending δ<sup>15</sup>N analyses of amino acids to bathypelagic and abyssopelagic depths for the first time, we confirmed that most particle remineralization and reworking occurs within the upper ∼400 m of the water column regardless of initial surface productivity. At Station ALOHA, we further used 16S rRNA barcoding to characterize the microbial communities associated with the POM. We found that chemolithoautotrophic ammonia-oxidizing archaea are abundant in the upper water column at Station ALOHA and that their abundance corresponded to regions of high heterotrophic reworking as indicated by amino acid isotope analysis.</div></div>","PeriodicalId":51009,"journal":{"name":"Deep-Sea Research Part I-Oceanographic Research Papers","volume":"225 ","pages":"Article 104597"},"PeriodicalIF":2.1000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transformations of particulate organic matter from the surface to the abyssal plain in the North Pacific as inferred from compound-specific stable isotope and microbial community analyses\",\"authors\":\"Lee C. Miller , Hilary G. Close , Kalina C. Grabb , Christine L. Huffard , Fuyan Li , David M. Karl , Kenneth L. Smith , Edward F. DeLong , Claudia R. Benitez-Nelson , Jeffrey C. Drazen , Brian N. Popp\",\"doi\":\"10.1016/j.dsr.2025.104597\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Particulate organic matter (POM) produced in surface waters undergoes extensive reworking and breakdown by microbial and metazoan communities as it sinks to the abyssal seafloor and serves as the base of benthic and pelagic food webs. Here, we examined how various size classes of POM in the oligotrophic North Pacific Subtropical Gyre (Station ALOHA) and in the eutrophic California Current System (Station M) undergo microbial alteration throughout the water column. Compound-specific stable isotope analysis showed that sampling method strongly impacts the type of POM quantified as export to the deep sea. Moored abyssal sediment traps captured material that matched the isotopic composition of surface POM, indicating they collected large, fast-sinking particles, in contrast to the more heavily reworked particles collected with <em>in situ</em> filtration at the same depths. Extending δ<sup>15</sup>N analyses of amino acids to bathypelagic and abyssopelagic depths for the first time, we confirmed that most particle remineralization and reworking occurs within the upper ∼400 m of the water column regardless of initial surface productivity. At Station ALOHA, we further used 16S rRNA barcoding to characterize the microbial communities associated with the POM. We found that chemolithoautotrophic ammonia-oxidizing archaea are abundant in the upper water column at Station ALOHA and that their abundance corresponded to regions of high heterotrophic reworking as indicated by amino acid isotope analysis.</div></div>\",\"PeriodicalId\":51009,\"journal\":{\"name\":\"Deep-Sea Research Part I-Oceanographic Research Papers\",\"volume\":\"225 \",\"pages\":\"Article 104597\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Deep-Sea Research Part I-Oceanographic Research Papers\",\"FirstCategoryId\":\"89\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0967063725001554\",\"RegionNum\":3,\"RegionCategory\":\"地球科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"OCEANOGRAPHY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Deep-Sea Research Part I-Oceanographic Research Papers","FirstCategoryId":"89","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0967063725001554","RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OCEANOGRAPHY","Score":null,"Total":0}
Transformations of particulate organic matter from the surface to the abyssal plain in the North Pacific as inferred from compound-specific stable isotope and microbial community analyses
Particulate organic matter (POM) produced in surface waters undergoes extensive reworking and breakdown by microbial and metazoan communities as it sinks to the abyssal seafloor and serves as the base of benthic and pelagic food webs. Here, we examined how various size classes of POM in the oligotrophic North Pacific Subtropical Gyre (Station ALOHA) and in the eutrophic California Current System (Station M) undergo microbial alteration throughout the water column. Compound-specific stable isotope analysis showed that sampling method strongly impacts the type of POM quantified as export to the deep sea. Moored abyssal sediment traps captured material that matched the isotopic composition of surface POM, indicating they collected large, fast-sinking particles, in contrast to the more heavily reworked particles collected with in situ filtration at the same depths. Extending δ15N analyses of amino acids to bathypelagic and abyssopelagic depths for the first time, we confirmed that most particle remineralization and reworking occurs within the upper ∼400 m of the water column regardless of initial surface productivity. At Station ALOHA, we further used 16S rRNA barcoding to characterize the microbial communities associated with the POM. We found that chemolithoautotrophic ammonia-oxidizing archaea are abundant in the upper water column at Station ALOHA and that their abundance corresponded to regions of high heterotrophic reworking as indicated by amino acid isotope analysis.
期刊介绍:
Deep-Sea Research Part I: Oceanographic Research Papers is devoted to the publication of the results of original scientific research, including theoretical work of evident oceanographic applicability; and the solution of instrumental or methodological problems with evidence of successful use. The journal is distinguished by its interdisciplinary nature and its breadth, covering the geological, physical, chemical and biological aspects of the ocean and its boundaries with the sea floor and the atmosphere. In addition to regular "Research Papers" and "Instruments and Methods" papers, briefer communications may be published as "Notes". Supplemental matter, such as extensive data tables or graphs and multimedia content, may be published as electronic appendices.