数据显示resolvin D5对前列腺素E2介导的fMet-Leu-Phe诱导的人中性粒细胞NADPH氧化酶活化的抑制作用

IF 1.4 Q3 MULTIDISCIPLINARY SCIENCES

Data in Brief Pub Date : 2025-09-19 DOI:10.1016/j.dib.2025.112080

Wenyan Li , Claes Dahlgren , Huamei Forsman

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引用次数: 0

摘要

产生NADPH氧化酶的活性氧（ROS）的调节，在中性粒细胞中表达，对于促炎抗微生物宿主防御和限制组织破坏炎症过程的解决反应性之间的平衡是必不可少的。采用标准的密度梯度离心分离法，从Sahlgrenska大学医院血库中获得的黄皮中分离出健康成人的外周血中性粒细胞。中性粒细胞被甲酰基肽受体1 （FPR1）识别的肽fMet-Leu-Phe （fMLF）激活。占据FPR1的激动剂下游产生的信号激活中性粒细胞质膜中的NADPH氧化酶。使用非常敏感的异醇扩增化学发光系统实时测量fMLF激活的中性粒细胞释放的ROS，以光单位（兆计数每分钟；Mcpm）表示，并确定响应的峰值水平。FPR1产生的激活信号被占据的EP4（前列腺素E2 （PGE2）的中性粒细胞受体）激动剂抑制（峰值降低）。Resolvin D5 （RvD5）有望增强PGE2的抑制作用（正调节），RvD5是由omega-3脂肪酸二十二碳六烯酸产生的，是专门的促溶解脂类的成员。本文提供的数据集包括RvD5对PGE2抑制活性影响的原始数据。重复使用的数据表明，负变构调节剂对中性粒细胞中ROS的产生缺乏抑制作用，这在几个不同的研究领域是高度和多方面的，例如药物开发（药物作用在其他地方或应该重新评估）和免疫学研究（中性粒细胞研究和动物模型，并作为测试其他ROS检测分析或生物传感器的稳健性或特异性的参考）。

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Data showing effects of resolvin D5 on prostaglandin E2 mediated inhibition of fMet-Leu-Phe induced activation of the NADPH oxidase in human neutrophils

Regulation of the reactive oxygen species (ROS) producing NADPH oxidase, expressed in neutrophils, is essential for a balance between the proinflammatory antimicrobial host defence and the resolving reactivity that limits tissue destructing inflammatory processes. Peripheral blood neutrophils of healthy adults were isolated from buffy coats obtained from the blood bank at Sahlgrenska University Hospital, using a standard density-gradient centrifugation protocol. The neutrophils were activated by fMet-Leu-Phe (fMLF) a peptide recognized by formyl peptide receptor 1 (FPR1). Signals generated downstream of the agonist occupied FPR1 activate the NADPH oxidase in the neutrophil plasma membrane. The release of ROS by fMLF activated neutrophils was measured in real time using a very sensitive isoluminol-amplified chemiluminescence system, expressed in light units (Mega counts per minute; Mcpm), and the peak value levels of the responses were determined. The activation signals generated by FPR1 were inhibited (reduced peak values) by the agonist occupied EP₄, a neutrophil receptor for prostaglandin E₂ (PGE₂). The inhibitory effect of PGE₂ was expected to be increased (positively modulated) by Resolvin D₅ (RvD₅), generated from the omega-3 fatty acid docosahexaenoic acid and a member of the group of specialized pro-resolving lipids. The dataset presented in the article includes raw data on the effects of RvD₅ on the inhibitory activity of PGE₂. The reuse of data indicating a lack of inhibition of a negative allosteric modulator on ROS production in neutrophils is high and multifaceted across several different research domains such as drug development (drug effects lies elsewhere or should be re-evaluated) and immunological research (neutrophil studies and animal models and as reference for testing the robustness or specificity of other ROS-detecting assays or biosensors).

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来源期刊

Data in Brief MULTIDISCIPLINARY SCIENCES-

CiteScore

3.10

自引率

0.00%

发文量

996

审稿时长

70 days

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