血浆氨基酸谱测定作为儿科轻度创伤性脑损伤患者潜在生物标志物的生物学可行性

IF 3 Q2 CLINICAL NEUROLOGY
Adán Pérez-Arredondo, Eduardo Cázares-Ramírez, Luis Tristán-López, Carlos Jiménez-Gutiérrez, Diana L Pérez-Lozano, Ivette A Martínez-Hernández, Valentina Vega-Rangel, Hugo F Narváez-González, Camilo Rios, Marina Martínez-Vargas, Luz Navarro, Liliana Carmona-Aparicio
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引用次数: 0

摘要

背景:氨基酸生物标志物对我们理解脑损伤机制有重要影响,它们的血浆浓度可能指示神经损伤和恢复模式。儿科轻度创伤性脑损伤(mTBI)评估尤其受益于这些分子指标,因为临床表现可能是微妙和可变的。然而,目前的诊断和预后工具缺乏可靠的生化标志物来跟踪损伤和恢复的时间演变。方法:我们进行了一项前瞻性纵向队列研究,涉及36名儿童mTBI患者和44名对照组,以表征关键氨基酸及其衍生指标的时间演变。分别于伤后3、6、12、24 h和7、14、28 d采集血样,用高效液相色谱法测定氨基酸含量。结果:我们的分析揭示了谷氨酸、谷氨酰胺和甘氨酸浓度的显著时间变化,谷氨酸在第7天达到峰值,然后下降,而谷氨酰胺在整个过程中稳步上升。GLN/GLU比值表现为早期兴奋性失衡,随后出现星形细胞代偿,GLX比值表现为逐渐恢复。结论:这些模式代表了涉及兴奋毒性和胶质调节的连续神经化学过程,表明作为mTBI诊断和监测的生物标志物的潜在效用。虽然需要使用更大的队列进行进一步验证,但这些发现为使用氨基酸谱追踪儿童mTBI进展和恢复的有效性提供了令人信服的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biological Plausibility of Using Plasma Amino Acid Profile Determination as a Potential Biomarker for Pediatric Patients with Mild Traumatic Brain Injuries.

Background: Amino acid biomarkers have a crucial influence on our understanding of brain injury mechanisms, and their plasma concentrations may indicate neurological damage and recovery patterns. Pediatric mild traumatic brain injury (mTBI) assessment particularly benefits from such molecular indicators, as clinical presentations can be subtle and variable. However, current diagnostic and prognostic tools lack reliable biochemical markers that can track the temporal evolution of injuries and recovery.

Methods: We conducted a prospective longitudinal cohort study involving 36 pediatric mTBI patients and 44 controls to characterize the temporal evolution of key amino acids and their derived indices. Blood samples were collected at 3, 6, 12, and 24 h and at 7, 14, and 28 days post-injury, with amino acids quantified using high-performance liquid chromatography.

Results: Our analysis revealed significant temporal changes in glutamate, glutamine, and glycine concentrations, with glutamate peaking at day 7 before declining, while glutamine showed steady increases throughout. The GLN/GLU ratio demonstrated an early excitatory imbalance followed by astrocytic compensation, and the GLX ratio indicated progressive recovery.

Conclusions: These patterns represent continuous neurochemical processes involving excitotoxicity and glial regulation, suggesting potential utility as biomarkers for mTBI diagnosis and monitoring. While further validation using larger cohorts is needed, these findings provide compelling evidence of the efficacy of using amino acid profiles to track pediatric mTBI progression and recovery.

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来源期刊
Neurology International
Neurology International CLINICAL NEUROLOGY-
CiteScore
3.70
自引率
3.30%
发文量
69
审稿时长
11 weeks
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