一种用于黑足三趾鹬分子性别鉴定的改良引物对。

IF 0.9 4区环境科学与生态学 Q4 BIODIVERSITY CONSERVATION

Conservation Genetics Resources Pub Date : 2025-01-01 Epub Date: 2025-07-02 DOI:10.1007/s12686-025-01400-x

Chloe P Cargill, George Harrand, Signe Christensen-Dalsgaard, Alexey V Ezhov, Ruben C Fijn, Maria V Gavrilo, Mark A Newell, Carmel McDougall

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引用次数: 0

摘要

在鸟类种群的研究中，准确的性别识别有助于研究与性别相关的行为、生态、社会结构、决策和生活史。为了改进黑腿三趾鸥（Laridae: Rissa tridactyla，以下简称“三趾鸥”）的分子性别鉴定方案，将先前为其他鸟类开发的引物与三趾鸥CHD1 Z和W染色体重新组合，以产生物种特异性引物对。采用典型的PCR和凝胶电泳方法分别获得602 bp和375 bp的扩增子。利用头喙长度对来自大西洋4个繁殖群体的138只三趾鸥进行了先验性鉴定，并对改进的引物对进行了验证。

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A modified primer-pair designed for the molecular sex identification of black-legged Kittiwake (Laridae: Rissa tridactyla).

In studies of avian populations accurate sex identification facilitates the study of sex-linked behaviour, ecology, social structure, decision-making, and life histories. To improve molecular sex identification protocols for the black-legged kittiwake (Laridae: Rissa tridactyla, hereafter 'kittiwake'), primers previously developed for other bird species were realigned against the kittiwake CHD1 Z and W chromosomes to generate a species-specific primer-pair. Amplicons of 602 bp and 375 bp were produced using typical PCR and gel electrophoresis procedures. The modified primer-pair was validated on a test sample of 138 kittiwakes from four Atlantic breeding colonies sexed a priori using head-bill length.

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来源期刊

Conservation Genetics Resources BIODIVERSITY CONSERVATION-GENETICS & HEREDITY

CiteScore

2.90

自引率

9.10%

发文量

审稿时长

3-6 weeks

期刊介绍： Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.