大豆分离蛋白与EGCG和茶多酚的相互作用:对蛋白质结构和豆类主要风味物质结合的影响

IF 3.4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Tianyu Zhang, Lingyu Yang, Xiangquan Zeng, Wentao Zhang, Zhiwei Ying, Xinqi Liu, He Li
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引用次数: 0

摘要

本研究研究了不同浓度的表没食子儿茶素没食子酸酯(EGCG)和茶多酚处理对大豆分离蛋白(SPI)的影响,特别关注了SPI的结构修饰及其随后与特征豆味化合物的相互作用。EGCG和茶多酚处理显著降低了SPI对特色风味化合物(己醛、苯甲醛、1-辛烯-3-醇和2-壬酮)的吸附能力,同时显著增强了对2-戊基呋喃的吸附能力。EGCG/茶多酚处理提高了SPI的溶解度,增强了荧光猝灭效果。此外,作为EGCG, SPI中的α-螺旋和β-转结构转变为β-片状和随机线圈状结构,降低了表面疏水性。分子对接结果表明,SPI与EGCG或茶多酚之间的相互作用主要是氢键和疏水相互作用。感官评价表明,EGCG和茶多酚能有效地减轻SPI的脂肪味特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The Interaction Between Soybean Protein Isolate and EGCG and Tea Polyphenols: Effects on Protein Structure and Binding of Key Beany Flavor Substances

The Interaction Between Soybean Protein Isolate and EGCG and Tea Polyphenols: Effects on Protein Structure and Binding of Key Beany Flavor Substances

This study investigated the effects of epigallocatechin gallate (EGCG) and tea polyphenol treatments at varying concentrations on soybean protein isolate (SPI), with particular focus on SPI's structural modifications and its subsequent interactions with characteristic beany flavor compounds. Treatment with EGCG and tea polyphenols significantly decreased SPI's adsorption capacity for characteristic beany flavor compounds (hexanal, benzaldehyde, 1-octen-3-ol, and 2-nonanone), while markedly enhancing adsorption of 2-pentylfuran. EGCG/tea polyphenol treatment improved the solubility of SPI and enhanced the fluorescence quenching effect. Additionally, as EGCG, the α-helix and β-turn structure in SPI changed to a β-sheet and random coil structure, reducing surface hydrophobicity. The molecular docking results indicate that the interactions between SPI and EGCG or tea polyphenols are primarily hydrogen bonding and hydrophobic interactions. The sensory evaluation demonstrated that EGCG and tea polyphenols effectively mitigated the unpleasant fatty flavor characteristics of SPI.

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来源期刊
Journal of Food Science
Journal of Food Science 工程技术-食品科技
CiteScore
7.10
自引率
2.60%
发文量
412
审稿时长
3.1 months
期刊介绍: The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.
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