Green Synthesis Silver Nanoparticles Based on Polysaccharides Extract of Amanita pantherina: Electrochemical Characteristics and Antibacterial Activity

Mushroom polysaccharides are biologically active substances with strong reduction, capture, and stabilization capabilities. They have great potential in the synthesis of metal nanoparticles. This study developed a method for the electrochemical synthesis of silver nanoparticles from polysaccharide extracts (AgNP-AcPOLY) of the Amanita pantherina mushroom. Silver nanoparticles (AgNPs) were characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), electrophoretic light scattering (ELS), UV-vis spectroscopic (UV-Vis), and laser diffraction spectroscopy (LDS) methods. XRD and TEM studies showed that the silver nanoparticles are spherical with a size of about 11.6 nm. The electrochemical performance of AgNP-AcPOLY nanoparticles was evaluated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results indicate that AgNP-AcPOLY exhibits outstanding electrochemical properties, significantly enhancing the performance of screen-printed electrodes (SPE). In addition, the antibacterial activity of nanosilver was evaluated through the methods of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against two strains of bacteria, Staphylococcus aureus and Escherichia coli. The results of this study indicate that the silver nanoparticles synthesized from the polysaccharide extract of A. pantherina are clean and environmentally friendly and have broad-spectrum antibacterial ability.