利用红外飞秒激光脉冲对胚胎滋养外胚层细胞进行活检

IF 0.7 4区 物理与天体物理 Q4 PHYSICS, MULTIDISCIPLINARY
D. S. Sitnikov, M. A. Filatov
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引用次数: 0

摘要

毫秒激光源现在广泛应用于辅助生殖技术,用于胚泡阶段的滋养外胚层细胞活检。我们研究了红外飞秒激光脉冲(持续时间为280fs,辐射波长为1028nm)在这方面应用的有效性。活检使用标准的显微外科辅助装置-手持移液器进行。脉冲强度为10至65 TW/cm2,能够从不同厚度的生物酸盐中提取细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biopsy of Embryonic Trophectoderm Cells Using Infrared Femtosecond Laser Pulses

Biopsy of Embryonic Trophectoderm Cells Using Infrared Femtosecond Laser Pulses

Millisecond laser sources are now widely used in assisted reproductive technologies for the trophectoderm cell biopsy procedure at the blastocyst stage. We have investigated the effectiveness of application for this purpose of infrared femtosecond laser pulses (280 fs duration and 1028 nm radiation wavelength). The biopsy was performed using standard microsurgical assist devices—holding pipettes. Pulses with an intensity of 10 to 65 TW/cm2 were shown to be able to extract cells from bioptates of different thickness.

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来源期刊
Bulletin of the Lebedev Physics Institute
Bulletin of the Lebedev Physics Institute PHYSICS, MULTIDISCIPLINARY-
CiteScore
0.70
自引率
25.00%
发文量
41
审稿时长
6-12 weeks
期刊介绍: Bulletin of the Lebedev Physics Institute is an international peer reviewed journal that publishes results of new original experimental and theoretical studies on all topics of physics: theoretical physics; atomic and molecular physics; nuclear physics; optics; lasers; condensed matter; physics of solids; biophysics, and others.
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