单核转录组学揭示了生长素驱动的木材可塑性增强杨树抗旱性的机制

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Daniela Gómez-Soto, Wendell J. Pereira, Alejandro Piedrabuena-Díaz, Christopher Dervinis, Matias Kirst, Isabel Allona, Mariano Perales, Daniel Conde
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引用次数: 0

摘要

干旱限制了森林和木本作物的生长,增加了它们对疾病的易感性,降低了它们的生产力,从而对它们产生了重大影响。木材的解剖可塑性是一种重要的适应机制,使树木能够应对水分供应的波动。在严重干旱期间,树木会形成更多更窄的血管,从而提高水力安全性并降低栓塞风险。然而,血管形成的分子调控尚不清楚。利用单核转录组学技术,建立了杨树成熟茎秆在水分充足和干旱条件下的细胞特异性基因表达图谱。我们的研究结果揭示了在木质部形成细胞中广泛的基因表达重编程,生长素稳态的变化被认为是解剖适应的关键机制。具体来说,我们表明杨树wat1样基因控制血管空间模式。此外,在干旱条件下,维管形成层分裂细胞中WAT1-like基因表达的下调和早期导管分化细胞中MP-like基因表达的上调促进了次生木质部血管变窄、数量增多的形成。此外,wat2突变体比野生型树木表现出更强的耐旱性,强调了其开发抗旱树种的潜力。本研究首次获得了严重干旱条件下杂交杨树茎的单核转录组图谱,揭示了调控木质部可塑性和增强抗旱性的生长素驱动激素网络。这些见解为提高杨树和其他木本树种的抗逆性提供了有价值的目标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Single-nucleus transcriptomics revealed auxin-driven mechanisms of wood plasticity to enhance severe drought tolerance in poplar
Drought significantly affects forests and woody crops by limiting their growth, increasing their susceptibility to diseases, and reducing productivity. Wood anatomical plasticity is a crucial adaptive mechanism that enables trees to cope with fluctuations in water availability. During severe drought, trees develop more and narrower vessels, enhancing hydraulic safety and reducing the risk of embolism. However, the molecular regulation of vessel formation is still not well understood. Using single-nucleus transcriptomics, we have generated a cell type-specific gene expression map of the mature poplar stem under well-watered and drought conditions. Our findings reveal extensive gene expression reprogramming in xylem-forming cells, with changes in auxin homeostasis identified as a key mechanism for anatomical adaptation. Specifically, we show that poplar WAT1-like genes control vessel spatial patterning. Additionally, the downregulation of WAT1-like gene expression in the dividing cells of the vascular cambium and the upregulation of MP-like gene expression in cells undergoing early vessel differentiation facilitate the formation of secondary xylem with narrower and more numerous vessels under drought. Furthermore, the wat2 mutant exhibits greater drought tolerance than wild-type trees, underscoring its potential for developing drought-resilient tree varieties. This study provides the first single-nucleus transcriptomic map of hybrid poplar stems under severe drought, uncovering auxin-driven hormonal networks that regulate xylem plasticity and enhance drought tolerance. These insights provide valuable targets for improving resilience in poplar and other woody species.
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来源期刊
Genome Biology
Genome Biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
21.00
自引率
3.30%
发文量
241
审稿时长
2 months
期刊介绍: Genome Biology stands as a premier platform for exceptional research across all domains of biology and biomedicine, explored through a genomic and post-genomic lens. With an impressive impact factor of 12.3 (2022),* the journal secures its position as the 3rd-ranked research journal in the Genetics and Heredity category and the 2nd-ranked research journal in the Biotechnology and Applied Microbiology category by Thomson Reuters. Notably, Genome Biology holds the distinction of being the highest-ranked open-access journal in this category. Our dedicated team of highly trained in-house Editors collaborates closely with our esteemed Editorial Board of international experts, ensuring the journal remains on the forefront of scientific advances and community standards. Regular engagement with researchers at conferences and institute visits underscores our commitment to staying abreast of the latest developments in the field.
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