{"title":"大鼠细胞系鉴定16位点STR方法的建立与应用","authors":"Meimei Yang, Ting Xu, Nan Li, Yidan Sun, Wei Huang, Xuanyi Liang, Chao Shen","doi":"10.1096/fj.202501145R","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Cell line cross-contamination has been a longstanding issue in cell biology, with short tandem repeat (STR) analysis widely regarded as the gold standard for cell line authentication. However, until now, there have been few reports focused on authenticating rat cell lines. In a significant advancement, we have developed a 16-loci STR method specifically for rat cell line authentication. Using this method, we successfully distinguished 26 different rat cell lines, demonstrating the high resolution and reliability of our approach. Furthermore, we have generated and made publicly available a comprehensive rat STR database, which is unprecedented in the field. This database facilitates data comparison and validation, providing researchers with a critical resource to ensure the accuracy and integrity of their cell-based studies. Our work marks the first report on a dedicated rat STR method and the establishment of a corresponding STR database, representing a major step forward in advancing the reliability and reproducibility of rat cell line research. This development not only enhances the ability to authenticate rat cell lines but also sets a new benchmark for maintaining the purity and validity of experimental outcomes in this important model organism.</p>\n </div>","PeriodicalId":50455,"journal":{"name":"The FASEB Journal","volume":"39 19","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Establishment and Application of a 16-Loci STR Method for Rat Cell Line Authentication\",\"authors\":\"Meimei Yang, Ting Xu, Nan Li, Yidan Sun, Wei Huang, Xuanyi Liang, Chao Shen\",\"doi\":\"10.1096/fj.202501145R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Cell line cross-contamination has been a longstanding issue in cell biology, with short tandem repeat (STR) analysis widely regarded as the gold standard for cell line authentication. However, until now, there have been few reports focused on authenticating rat cell lines. In a significant advancement, we have developed a 16-loci STR method specifically for rat cell line authentication. Using this method, we successfully distinguished 26 different rat cell lines, demonstrating the high resolution and reliability of our approach. Furthermore, we have generated and made publicly available a comprehensive rat STR database, which is unprecedented in the field. This database facilitates data comparison and validation, providing researchers with a critical resource to ensure the accuracy and integrity of their cell-based studies. Our work marks the first report on a dedicated rat STR method and the establishment of a corresponding STR database, representing a major step forward in advancing the reliability and reproducibility of rat cell line research. This development not only enhances the ability to authenticate rat cell lines but also sets a new benchmark for maintaining the purity and validity of experimental outcomes in this important model organism.</p>\\n </div>\",\"PeriodicalId\":50455,\"journal\":{\"name\":\"The FASEB Journal\",\"volume\":\"39 19\",\"pages\":\"\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The FASEB Journal\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202501145R\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The FASEB Journal","FirstCategoryId":"99","ListUrlMain":"https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202501145R","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Establishment and Application of a 16-Loci STR Method for Rat Cell Line Authentication
Cell line cross-contamination has been a longstanding issue in cell biology, with short tandem repeat (STR) analysis widely regarded as the gold standard for cell line authentication. However, until now, there have been few reports focused on authenticating rat cell lines. In a significant advancement, we have developed a 16-loci STR method specifically for rat cell line authentication. Using this method, we successfully distinguished 26 different rat cell lines, demonstrating the high resolution and reliability of our approach. Furthermore, we have generated and made publicly available a comprehensive rat STR database, which is unprecedented in the field. This database facilitates data comparison and validation, providing researchers with a critical resource to ensure the accuracy and integrity of their cell-based studies. Our work marks the first report on a dedicated rat STR method and the establishment of a corresponding STR database, representing a major step forward in advancing the reliability and reproducibility of rat cell line research. This development not only enhances the ability to authenticate rat cell lines but also sets a new benchmark for maintaining the purity and validity of experimental outcomes in this important model organism.
期刊介绍：
The FASEB Journal publishes international, transdisciplinary research covering all fields of biology at every level of organization: atomic, molecular, cell, tissue, organ, organismic and population. While the journal strives to include research that cuts across the biological sciences, it also considers submissions that lie within one field, but may have implications for other fields as well. The journal seeks to publish basic and translational research, but also welcomes reports of pre-clinical and early clinical research. In addition to research, review, and hypothesis submissions, The FASEB Journal also seeks perspectives, commentaries, book reviews, and similar content related to the life sciences in its Up Front section.
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