{"title":"FAM163B的神经特异性表达和膜定位调控SAP97的表达","authors":"Xiaoyu Zhang, Shanshan Sun, Wei Zhao, Yan Jiang, Yuting Weng, Ilgiz Gareev, Xuemin Ge, Xinrong Li, Jiaqi Wang, Qiongqiong Hu, Dechun Cheng, Lili Mu, Jinghua Wang","doi":"10.1111/jnc.70244","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The cell membrane plays a significant role in the development and functional maintenance of neurons. However, the functions of many neuronal membrane proteins remain unclear, posing a challenge to the treatment of brain diseases. Family with sequence similarity 163 member B (FAM163B) is a single-transmembrane protein, and <i>FAM163B</i> is a neuroregulatory gene with unknown functions. In this study, we aimed to elucidate the function of FAM163B in neurons of the mouse brain. FAM163B was exclusively expressed in the mouse brain among various tissues analyzed. Immunoblotting, real-time PCR, and cellular immunofluorescence confirmed high expression of FAM163B in mouse neurons, which were mainly located in the cell membrane. Overexpression of FAM163B in SH-SY5Y cells and primary mouse cortical neurons resulted in downregulated expression of SAP97, and knockdown of FAM163B increased the expression level of SAP97. SAP97 is an important transporter that plays a part in synaptic function and neuroplasticity. Besides, an interaction was found between FAM163B and VAPA. VAPA is involved in the intracellular vesicle transport and membrane fusion processes. By staining with FM4-64FX, overexpression of FAM163B weakened the endocytosis ability of neurons, while knockdown enhanced it. The study provides for an understanding of the roles of FAM163B in neurons.\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>\n </div>","PeriodicalId":16527,"journal":{"name":"Journal of Neurochemistry","volume":"169 10","pages":""},"PeriodicalIF":4.0000,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Neural-Specific Expression and Membrane Localization of FAM163B Regulates SAP97 Expression\",\"authors\":\"Xiaoyu Zhang, Shanshan Sun, Wei Zhao, Yan Jiang, Yuting Weng, Ilgiz Gareev, Xuemin Ge, Xinrong Li, Jiaqi Wang, Qiongqiong Hu, Dechun Cheng, Lili Mu, Jinghua Wang\",\"doi\":\"10.1111/jnc.70244\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>The cell membrane plays a significant role in the development and functional maintenance of neurons. However, the functions of many neuronal membrane proteins remain unclear, posing a challenge to the treatment of brain diseases. Family with sequence similarity 163 member B (FAM163B) is a single-transmembrane protein, and <i>FAM163B</i> is a neuroregulatory gene with unknown functions. In this study, we aimed to elucidate the function of FAM163B in neurons of the mouse brain. FAM163B was exclusively expressed in the mouse brain among various tissues analyzed. Immunoblotting, real-time PCR, and cellular immunofluorescence confirmed high expression of FAM163B in mouse neurons, which were mainly located in the cell membrane. Overexpression of FAM163B in SH-SY5Y cells and primary mouse cortical neurons resulted in downregulated expression of SAP97, and knockdown of FAM163B increased the expression level of SAP97. SAP97 is an important transporter that plays a part in synaptic function and neuroplasticity. Besides, an interaction was found between FAM163B and VAPA. VAPA is involved in the intracellular vesicle transport and membrane fusion processes. By staining with FM4-64FX, overexpression of FAM163B weakened the endocytosis ability of neurons, while knockdown enhanced it. The study provides for an understanding of the roles of FAM163B in neurons.\\n <figure>\\n <div><picture>\\n <source></source></picture><p></p>\\n </div>\\n </figure></p>\\n </div>\",\"PeriodicalId\":16527,\"journal\":{\"name\":\"Journal of Neurochemistry\",\"volume\":\"169 10\",\"pages\":\"\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Neurochemistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jnc.70244\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Neurochemistry","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jnc.70244","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Neural-Specific Expression and Membrane Localization of FAM163B Regulates SAP97 Expression
The cell membrane plays a significant role in the development and functional maintenance of neurons. However, the functions of many neuronal membrane proteins remain unclear, posing a challenge to the treatment of brain diseases. Family with sequence similarity 163 member B (FAM163B) is a single-transmembrane protein, and FAM163B is a neuroregulatory gene with unknown functions. In this study, we aimed to elucidate the function of FAM163B in neurons of the mouse brain. FAM163B was exclusively expressed in the mouse brain among various tissues analyzed. Immunoblotting, real-time PCR, and cellular immunofluorescence confirmed high expression of FAM163B in mouse neurons, which were mainly located in the cell membrane. Overexpression of FAM163B in SH-SY5Y cells and primary mouse cortical neurons resulted in downregulated expression of SAP97, and knockdown of FAM163B increased the expression level of SAP97. SAP97 is an important transporter that plays a part in synaptic function and neuroplasticity. Besides, an interaction was found between FAM163B and VAPA. VAPA is involved in the intracellular vesicle transport and membrane fusion processes. By staining with FM4-64FX, overexpression of FAM163B weakened the endocytosis ability of neurons, while knockdown enhanced it. The study provides for an understanding of the roles of FAM163B in neurons.
期刊介绍：
Journal of Neurochemistry focuses on molecular, cellular and biochemical aspects of the nervous system, the pathogenesis of neurological disorders and the development of disease specific biomarkers. It is devoted to the prompt publication of original findings of the highest scientific priority and value that provide novel mechanistic insights, represent a clear advance over previous studies and have the potential to generate exciting future research.
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