陆地植物特异性H3K27甲基转移酶ATXR5和ATXR6控制植物发育和胁迫反应

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xiaoyi Li, Jie Pan, Qian Liu, Huairen Zhang, Hui Li, Danhua Jiang
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引用次数: 0

摘要

组蛋白修饰对转录调控至关重要。陆地植物中一个显著的遗传创新是组蛋白赖氨酸甲基转移酶ATXR5/6的出现,该酶特异性催化抑制组蛋白H3赖氨酸27单甲基化(H3K27me1)。目前对atxr5 /6功能的了解主要基于使用弱atxr5的拟南芥研究;atxr6亚型突变体,其中atxr6仍部分表达,缺陷主要在异染色质中观察到。然而,陆地植物进化这些酶的意义尚不清楚。在本研究中,我们生成了强atxr5;在拟南芥中进一步降低atxr6表达的atxr6突变体,以探索ATXR5/6的更广泛作用。研究结果表明,ATXR5/6在植物生殖发育中起着重要作用,并通过抑制胁迫应答基因的转录,在支持植物正常生长方面发挥着关键作用。此外,ATXR5/6对于维持H3K27三甲基化(H3K27me3)是必需的,可能是通过提供H3K27me1作为进一步甲基化的底物。我们还证明了ATXR5/6在单株水稻中调控发育和应答基因的功能是保守的。我们的研究结果表明,ATXR5/6在陆地植物进化过程中不仅具有维持异染色质的功能,还具有调节发育和环境响应的功能,这为ATXR5/6在陆地植物中的功能意义提供了新的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Land plant-specific H3K27 methyltransferases ATXR5 and ATXR6 control plant development and stress responses
Histone modifications are critical for transcriptional regulation. A notable genetic innovation in land plants is the emergence of histone lysine methyltransferases ATXR5/6, which specifically catalyze the repressive histone H3 lysine 27 monomethylation (H3K27me1). Current knowledge of ATXR5/6 function is largely based on Arabidopsis studies using a weak atxr5;atxr6 hypomorphic mutant, in which ATXR6 is still partially expressed and defects are primarily observed in heterochromatin. However, the significance for land plants to evolve these enzymes remains unclear. In this study, we generate strong atxr5;atxr6 mutants with further reduced ATXR6 expression in Arabidopsis to explore the broader roles of ATXR5/6. Our results show that ATXR5/6 are essential for plant reproductive development and play a critical role in supporting normal plant growth by repressing the transcription of stress responsive genes. In addition, ATXR5/6 are necessary for maintaining H3K27 trimethylation (H3K27me3), likely by providing H3K27me1 as a substrate for further methylation. We also demonstrate that the function of ATXR5/6 in regulating development and responsive genes is conserved in the monocot rice. Our findings suggest that land plants evolved ATXR5/6 not only to maintain heterochromatin, but also to regulate development and environmental responses, providing new insights into the functional significance of ATXR5/6 in land plants.
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来源期刊
Genome Biology
Genome Biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
21.00
自引率
3.30%
发文量
241
审稿时长
2 months
期刊介绍: Genome Biology stands as a premier platform for exceptional research across all domains of biology and biomedicine, explored through a genomic and post-genomic lens. With an impressive impact factor of 12.3 (2022),* the journal secures its position as the 3rd-ranked research journal in the Genetics and Heredity category and the 2nd-ranked research journal in the Biotechnology and Applied Microbiology category by Thomson Reuters. Notably, Genome Biology holds the distinction of being the highest-ranked open-access journal in this category. Our dedicated team of highly trained in-house Editors collaborates closely with our esteemed Editorial Board of international experts, ensuring the journal remains on the forefront of scientific advances and community standards. Regular engagement with researchers at conferences and institute visits underscores our commitment to staying abreast of the latest developments in the field.
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