猪模型部分厚度热烧伤后的即时和中期辅助创面治疗。

IF 1.3 4区 医学 Q3 OPHTHALMOLOGY
Donovan S Reed, Hunter Phillips, Joshua Hohlbein, Brett Davies, Wesley Brundridge, Tanuj Nakra, Vikram Durairaj
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引用次数: 0

摘要

目的:利用猪模型来确定几种目前可用的治疗方法对部分厚度热烧伤后伤口愈合的临床和组织学影响。方法:在猪局部厚度热烧伤模型中进行前瞻性研究,比较羊膜移植、肉毒杆菌毒素A注射、维替波芬注射液即刻辅助创面治疗与局部5-氟尿嘧啶微针、维替波芬注射液、曲安奈德注射液加5-氟尿嘧啶和不加5-氟尿嘧啶的5-氟尿嘧啶局部注射辅助创面治疗的效果。临床评估由盲法整形外科医生使用有效的猪烧伤疤痕量表进行。采用苏木精染色、伊红染色和马松三色染色进行组织学分析。进行重复测量混合模型方差分析以确定临床或组织学统计学意义。结果:直接治疗与中间治疗比较,损伤后第14天和第28天时间点的平均临床分级差异有统计学意义,分别产生0.57分差(p = 0.0096)和0.7分差(p = 0.0006)。在损伤后28天的时间点上,上皮增生的平均组织学分级降低具有统计学意义(-30.532,p值)。结论:本研究的结果表明,与在愈合中期使用辅助伤口治疗相比,立即使用辅助伤口治疗局部厚度热损伤可减少随后的烧伤瘢痕形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immediate and Intermediate Adjuvant Wound Therapy Following Partial-Thickness Thermal Burn Injury in a Porcine Model.

Purpose: A porcine model was utilized to determine the clinical and histological impact of several currently available therapies for wound healing modification following partial-thickness thermal burn injury.

Methods: A prospective study in a porcine model of partial-thickness thermal burn injury was performed, assessing immediate adjuvant wound therapy with amniotic membrane grafting, onabotulinum toxin A injection, and verteporfin injection compared with intermediate wound adjuvant therapy by intralesional 5-fluorouracil with microneedling, verteporfin injection, and triamcinolone injection with and without 5-fluorouracil. Clinical assessments were conducted by blinded plastic surgeons utilizing a validated porcine burn scar scale. Histologic analysis was accomplished with hematoxylin and eosin stains and Masson's trichrome stains. A repeated measures mixed model analysis of variance was performed to identify clinical or histological statistical significance.

Results: Comparing immediate versus intermediate treatments, a statistically significant difference in regards to mean clinical grade at the postinjury day 14 and postinjury day 28 time points was identified, yielding a 0.57 scale difference (p = 0.0096) and 0.7 scale difference (p = 0.0006), respectively. A statistically significant difference in regards to decreased mean histologic grade at the postinjury day 28 time points was identified for epithelial hyperplasia (-30.532 mean difference, p-value <0.0001), inflammatory response (-11.472 mean difference, p = 0.0133), and dermal remodeling (-13.78 mean difference, p = 0.0058).

Conclusions: The results of this study suggest immediate treatment of partial-thickness thermal injury with adjuvant wound therapy decreases subsequent burn scar formation when compared with adjuvant wound therapy applied in the intermediate phase of healing.

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来源期刊
CiteScore
2.50
自引率
10.00%
发文量
322
审稿时长
3-8 weeks
期刊介绍: Ophthalmic Plastic and Reconstructive Surgery features original articles and reviews on topics such as ptosis, eyelid reconstruction, orbital diagnosis and surgery, lacrimal problems, and eyelid malposition. Update reports on diagnostic techniques, surgical equipment and instrumentation, and medical therapies are included, as well as detailed analyses of recent research findings and their clinical applications.
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