Revolutionizing chlorogenic acid production: cutting-edge synthetic biology strategies

Chlorogenic acid, a bioactive compound with significant pharmacological and industrial value, is predominantly sourced through conventional extraction methods that exhibit resource dependency, high cost, inefficiencies, and environmental concerns. To address the dual challenges of surging market demand and environmental preservation, synthetic biology-driven microbial fermentation emerges as a pivotal sustainable strategy to overcome traditional production bottlenecks. Through rational design of metabolic networks, development of modular co-culture systems, and integration of intelligent regulation tools, efficient, resource-conserving, and environmentally benign chlorogenic acid production can be achieved. This study provides an innovative paradigm for the biomanufacturing of high-value chemicals.