Kyung-Ah Cho , Hyeon Ju Kim , SeoYi Choi , So-Youn Woo , Joo Young Roh
{"title":"表达信号蛋白7a的肥大细胞外泌体促进伤口愈合","authors":"Kyung-Ah Cho , Hyeon Ju Kim , SeoYi Choi , So-Youn Woo , Joo Young Roh","doi":"10.1016/j.yexcr.2025.114771","DOIUrl":null,"url":null,"abstract":"<div><div>Emerging evidence indicates that mast cells contribute to tissue repair, in addition to their well-established roles in immunity. We previously showed the effects of conditioned medium derived from the human mast cell line HMC-1 on skin wound healing. In this study, we assessed the regenerative potential of HMC-1 exosomes in the skin wound healing process and identified the key molecules involved. HMC-1 exosomes are rich in semaphorin 7A (SEMA7A), a key regulator of extracellular matrix (ECM) remodeling, along with canonical exosomal markers. We found that HMC-1 exosomes could promote dermal fibroblast proliferation and upregulate fibronectin and collagen type I expression. Furthermore, topical application of HMC-1 exosomes accelerated wound closure and enhanced tissue regeneration in both wild-type and mast cell-deficient mice. Notably, SEMA7A-deficient HMC-1 exosomes exhibited reduced ECM synthesis in dermal fibroblasts and impaired wound healing <em>in vivo</em>, regardless of the presence of mast cells. These findings suggest that mast cell-derived exosomes can serve as promising cell-free therapeutic agents for wound repair.</div></div>","PeriodicalId":12227,"journal":{"name":"Experimental cell research","volume":"452 2","pages":"Article 114771"},"PeriodicalIF":3.5000,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Semaphorin7A-expressing mast cell exosomes promote wound healing\",\"authors\":\"Kyung-Ah Cho , Hyeon Ju Kim , SeoYi Choi , So-Youn Woo , Joo Young Roh\",\"doi\":\"10.1016/j.yexcr.2025.114771\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Emerging evidence indicates that mast cells contribute to tissue repair, in addition to their well-established roles in immunity. We previously showed the effects of conditioned medium derived from the human mast cell line HMC-1 on skin wound healing. In this study, we assessed the regenerative potential of HMC-1 exosomes in the skin wound healing process and identified the key molecules involved. HMC-1 exosomes are rich in semaphorin 7A (SEMA7A), a key regulator of extracellular matrix (ECM) remodeling, along with canonical exosomal markers. We found that HMC-1 exosomes could promote dermal fibroblast proliferation and upregulate fibronectin and collagen type I expression. Furthermore, topical application of HMC-1 exosomes accelerated wound closure and enhanced tissue regeneration in both wild-type and mast cell-deficient mice. Notably, SEMA7A-deficient HMC-1 exosomes exhibited reduced ECM synthesis in dermal fibroblasts and impaired wound healing <em>in vivo</em>, regardless of the presence of mast cells. These findings suggest that mast cell-derived exosomes can serve as promising cell-free therapeutic agents for wound repair.</div></div>\",\"PeriodicalId\":12227,\"journal\":{\"name\":\"Experimental cell research\",\"volume\":\"452 2\",\"pages\":\"Article 114771\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2025-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental cell research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0014482725003714\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental cell research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014482725003714","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Emerging evidence indicates that mast cells contribute to tissue repair, in addition to their well-established roles in immunity. We previously showed the effects of conditioned medium derived from the human mast cell line HMC-1 on skin wound healing. In this study, we assessed the regenerative potential of HMC-1 exosomes in the skin wound healing process and identified the key molecules involved. HMC-1 exosomes are rich in semaphorin 7A (SEMA7A), a key regulator of extracellular matrix (ECM) remodeling, along with canonical exosomal markers. We found that HMC-1 exosomes could promote dermal fibroblast proliferation and upregulate fibronectin and collagen type I expression. Furthermore, topical application of HMC-1 exosomes accelerated wound closure and enhanced tissue regeneration in both wild-type and mast cell-deficient mice. Notably, SEMA7A-deficient HMC-1 exosomes exhibited reduced ECM synthesis in dermal fibroblasts and impaired wound healing in vivo, regardless of the presence of mast cells. These findings suggest that mast cell-derived exosomes can serve as promising cell-free therapeutic agents for wound repair.
期刊介绍:
Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.