表达信号蛋白7a的肥大细胞外泌体促进伤口愈合

IF 3.5 3区 生物学 Q3 CELL BIOLOGY
Kyung-Ah Cho , Hyeon Ju Kim , SeoYi Choi , So-Youn Woo , Joo Young Roh
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引用次数: 0

摘要

新出现的证据表明,肥大细胞除了在免疫系统中发挥既定作用外,还有助于组织修复。我们之前展示了从人肥大细胞系HMC-1提取的条件培养基对皮肤伤口愈合的影响。在这项研究中,我们评估了HMC-1外泌体在皮肤伤口愈合过程中的再生潜力,并确定了涉及的关键分子。hcc -1外泌体富含信号蛋白7A (SEMA7A),信号蛋白7A是细胞外基质(ECM)重塑的关键调节因子,以及典型的外泌体标记物。我们发现HMC-1外泌体可以促进真皮成纤维细胞增殖,上调纤维连接蛋白和I型胶原蛋白的表达。此外,HMC-1外泌体的局部应用加速了野生型和肥大细胞缺陷小鼠的伤口愈合和组织再生。值得注意的是,无论肥大细胞是否存在,缺乏sema7a的HMC-1外泌体在真皮成纤维细胞中表现出ECM合成减少和伤口愈合受损。这些发现表明肥大细胞来源的外泌体可以作为有前途的无细胞治疗剂用于伤口修复。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Semaphorin7A-expressing mast cell exosomes promote wound healing
Emerging evidence indicates that mast cells contribute to tissue repair, in addition to their well-established roles in immunity. We previously showed the effects of conditioned medium derived from the human mast cell line HMC-1 on skin wound healing. In this study, we assessed the regenerative potential of HMC-1 exosomes in the skin wound healing process and identified the key molecules involved. HMC-1 exosomes are rich in semaphorin 7A (SEMA7A), a key regulator of extracellular matrix (ECM) remodeling, along with canonical exosomal markers. We found that HMC-1 exosomes could promote dermal fibroblast proliferation and upregulate fibronectin and collagen type I expression. Furthermore, topical application of HMC-1 exosomes accelerated wound closure and enhanced tissue regeneration in both wild-type and mast cell-deficient mice. Notably, SEMA7A-deficient HMC-1 exosomes exhibited reduced ECM synthesis in dermal fibroblasts and impaired wound healing in vivo, regardless of the presence of mast cells. These findings suggest that mast cell-derived exosomes can serve as promising cell-free therapeutic agents for wound repair.
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来源期刊
Experimental cell research
Experimental cell research 医学-细胞生物学
CiteScore
7.20
自引率
0.00%
发文量
295
审稿时长
30 days
期刊介绍: Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.
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