唾液昼夜节律基因表达作为倒班工人早期认知障碍的分子指标:300名成人的横断面研究

IF 2.7 4区 医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Adam Karim, Youssef Rayan, Ziad Amir, Malik Samir, Tariq Jamal, Karim Adnan, Omar Fares, Kareem Zane, Samir Tarek, Amir Nasir, Rami Khalid, Zeyad Khalil, Yasin Rafiq, Idris Nader
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引用次数: 0

摘要

这项横断面研究探讨了唾液昼夜节律基因表达作为轮班工人早期认知障碍的非侵入性生物标志物的潜在效用。300名年龄在25至55岁之间的参与者被分为认知障碍轮班工作者(MoCA < 26, n = 100)、认知正常轮班工作者(n = 100)和非轮班工作对照组(n = 100)。分别于07:00和19:00采集唾液样本,采用qRT-PCR检测PER1、BMAL1和CLOCK mRNA的表达。有认知障碍的轮班工人在基因表达上的昼夜变化显著减弱,与两个对照组相比,夜间BMAL1和PER1水平降低(p < 0.001)。晚间BMAL1表达与认知状态独立相关(OR 2.14, 95% CI 1.62-2.85), AUC为0.876(敏感性81.3%,特异性78.0%)。联合三基因面板略微提高了诊断准确性(AUC 0.913)。这些初步研究结果表明,唾液昼夜节律基因表达的改变,特别是BMAL1,可能有望作为轮班工作人群早期神经认知变化的分子指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Salivary Circadian Gene Expression as a Molecular Indicator of Early Cognitive Impairment in Shift Workers: A Cross-Sectional Study of 300 Adults

This cross-sectional study explored the potential utility of salivary circadian gene expression as a non-invasive biomarker for early cognitive impairment in shift workers. Three hundred participants aged 25 to 55 were categorized into cognitively impaired shift workers (MoCA < 26, n = 100), cognitively intact shift workers (n = 100), and non-shift working controls (n = 100). Saliva samples collected at 07:00 and 19:00 were analyzed for mRNA expression of PER1, BMAL1, and CLOCK using qRT-PCR. Shift workers with cognitive impairment showed significantly attenuated diurnal variation in gene expression, with reduced evening levels of BMAL1 and PER1 compared to both control groups (p < 0.001). Evening BMAL1 expression was independently associated with cognitive status (OR 2.14, 95% CI 1.62–2.85), achieving an AUC of 0.876 (81.3% sensitivity, 78.0% specificity). A combined three-gene panel modestly improved diagnostic accuracy (AUC 0.913). These preliminary findings suggest that alterations in salivary circadian gene expression, particularly in BMAL1, may hold promise as a molecular indicator of early neurocognitive changes in shift-working populations.

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来源期刊
Journal of Molecular Neuroscience
Journal of Molecular Neuroscience 医学-神经科学
CiteScore
6.60
自引率
3.20%
发文量
142
审稿时长
1 months
期刊介绍: The Journal of Molecular Neuroscience is committed to the rapid publication of original findings that increase our understanding of the molecular structure, function, and development of the nervous system. The criteria for acceptance of manuscripts will be scientific excellence, originality, and relevance to the field of molecular neuroscience. Manuscripts with clinical relevance are especially encouraged since the journal seeks to provide a means for accelerating the progression of basic research findings toward clinical utilization. All experiments described in the Journal of Molecular Neuroscience that involve the use of animal or human subjects must have been approved by the appropriate institutional review committee and conform to accepted ethical standards.
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