Embryo Trophectoderm Cell Biopsy Using Femtosecond Laser Pulses

The use of millisecond laser sources has become an essential part of clinical practice in assisted reproductive technologies. The biopsy of embryos at the blastocyst stage is one of the challenges addressed in reproductive medicine. We have examined the feasibility of applying femtosecond laser pulses (with a duration of 280 fs and a radiation wavelength of 514 nm) and their effectiveness in solving this problem. The biopsy procedure was performed using micropipettes as part of a standard clinical protocol. It is demonstrated that pulses with an intensity of 4 to 6 TW/cm² can be used to separate bioptate cells. The outcomes of exposure in different regimes (from a single pulse to a sequence of pulses) are presented.