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引用次数: 0
摘要
将多重交叉位移扩增（MCDA）和基于金纳米颗粒的横向流动生物传感器（AuNPs-LFB）这两种可适用于医疗点（POC）的技术整合在一起，已经证明了对乙型肝炎病毒（HBV）和丙型肝炎病毒（HCV）的出色检测。在最近的一项研究中，Zhang等人（H. Zang， Y. Shi， Z. Wu， Q. Zhao, et ., Microbiol spectre 13:e01738- 24,2025, https://doi.org/10.1128/spectrum.01738-24）开发了一种针对HBV S基因和HCV 5'-UTR区域的新型单管多重MCDA-AuNPs-LFB检测方法。该分析在64°C的等温条件下运行35分钟，并且AuNPs-LFB分析允许对结果进行视觉解释。该平台的分析灵敏度与实验室定量PCR相当，但周转时间更短，成本更低，操作简单，对解剖相关的非目标病原体的交叉反应性不可检测，使其非常适合分散的POC诊断应用。混合系统在简化核酸检测方面的成功，同时增加了可移植性，表明临床诊断可能从以技术为中心的集中式实验室过渡到地方诊所、现场测试，甚至家庭护理。
The new gold rush in clinical diagnostics: from standard laboratory assays to fast and accurate point-of-care (POC) methods.
Integrating two point-of-care (POC)-amenable technologies, namely, multiple cross displacement amplification (MCDA) and gold nanoparticle-based lateral flow biosensors (AuNPs-LFB), has demonstrated excellent detection of hepatitis B virus (HBV) and hepatitis C virus (HCV). In a recent study, Zhang et al. (H. Zang, Y. Shi, Z. Wu, Q. Zhao, et al., Microbiol Spectr 13:e01738-24, 2025, https://doi.org/10.1128/spectrum.01738-24) developed a novel, single-tube, multiplex MCDA-AuNPs-LFB assay that targets the HBV S gene and the HCV 5'-UTR region. The assay operates under isothermal conditions at 64°C for 35 min, and the AuNPs-LFB assay permits a visual interpretation of results. This platform achieves an analytical sensitivity comparable to lab-based quantitative PCR but with shorter turnaround time, reduced cost, operational simplicity, and undetectable cross-reactivity to anatomically relevant, non-target pathogens, making it highly suitable for decentralized POC diagnostic applications. The success of hybrid systems in simplifying nucleic acid testing while increasing portability indicates a potential transition in clinical diagnosis from centralized tech-centered labs to local clinics, field testing, and even home care.
期刊介绍：
Microbiology Spectrum publishes commissioned review articles on topics in microbiology representing ten content areas: Archaea; Food Microbiology; Bacterial Genetics, Cell Biology, and Physiology; Clinical Microbiology; Environmental Microbiology and Ecology; Eukaryotic Microbes; Genomics, Computational, and Synthetic Microbiology; Immunology; Pathogenesis; and Virology. Reviews are interrelated, with each review linking to other related content. A large board of Microbiology Spectrum editors aids in the development of topics for potential reviews and in the identification of an editor, or editors, who shepherd each collection.
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