大肠杆菌脓毒症单核细胞分布宽度变化的比较分析:来自体内和离体模型的见解。

IF 3.7 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Daniela Ligi, Chiara Della Franca, Michela Pelloso, Alicia Martinez-Iribarren, Alba Leis, Erica Fabbri, Francesca Salvatori, Elena A Sukhacheva, Giorgio Brandi, Giuditta F Schiavano, Ferdinando Mannello
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引用次数: 0

摘要

目的:单核细胞分布宽度(MDW)是衡量大规模感染中单核细胞异质性的早期脓毒症指标。在体外脓毒症模型中,我们比较了大肠杆菌脓毒症患者的MDW变化与活的大肠杆菌和脂多糖的影响。我们还研究了脓毒症模型中单核细胞形态功能和炎症反应的动力学。方法:用活大肠杆菌(106-1010 CFU/mL)和LPS(0.1-10 μg/mL)体外刺激健康受试者全血。使用DxH 690T血液学分析仪(Beckman Coulter)在不同时间点评估全血细胞计数,包括MDW。回顾性比较败血症患者(n=23)的MDW值。采用数字细胞形态学(CellaVision DM软件)分析mayo - grunwald - giemsa染色的血涂片。定量血浆中27种炎症介质(Bio-Plex 200)。结果:活体大肠杆菌和LPS处理(离体模型)使MDW值早期显著升高,且呈剂量依赖性和时间依赖性。治疗后,IL-1β、TNF-α、IL-8、MIP1-α、MIP-1β、Eotaxin、G-CSF、PDGF-bb均显著升高。结论:我们的研究结果证实了MDW在脓毒症诊断中的临床应用,并维持了全血法作为离体脓毒症模型的可靠性。大肠杆菌和LPS直接促进早期单核细胞形态功能改变,反映在高MDW值和促炎介质上。这些结果提高了对脓毒症生物学基础的认识,为MDW在脓毒症中的有用性提供了新的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative analysis of monocyte distribution width alterations in Escherichia coli sepsis: insights from in vivo and ex vivo models.

Objectives: Monocyte distribution width (MDW) is an early sepsis indicator measuring monocyte heterogeneity during massive infection. We compared MDW changes in Escherichia coli sepsis patients with the effects of living E. coli and lipopolysaccharide in an ex vivo sepsis model. We also investigated the dynamics of monocyte morpho-functional and inflammatory responses in the sepsis model.

Methods: Whole blood from healthy participants was in vitro stimulated with live E. coli (106-1010 CFU/mL) and LPS (0.1-10 μg/mL). Complete blood counts, including MDW, were evaluated at different time-points using DxH 690T Hematology Analyzer (Beckman Coulter). MDW values were compared with those retrospectively obtained from sepsis patients (n=23). May-Grunwald-Giemsa-stained blood smears were analyzed by digital cell morphology (CellaVision DM software). A panel of 27 inflammatory mediators was quantified in plasma (Bio-Plex 200).

Results: MDW values were early and significantly increased in a dose- and time-dependent manner by live E. coli and LPS treatments (p<0.01). MDW values were significantly higher in sepsis patients compared to controls and overlapped those observed in the ex vivo model. IL-1β, TNF-α, IL-8, MIP1-α, MIP-1β, Eotaxin, G-CSF, and PDGF-bb were significantly modulated after treatments.

Conclusions: Our findings confirm the clinical utility of MDW in sepsis diagnosis and sustain the reliability of the whole blood assay as ex vivo sepsis model. E. coli and LPS directly promote early monocyte morpho-functional modifications, mirrored by high MDW values and pro-inflammatory mediators. These results improve the knowledge on the biological basis of sepsis, providing novel evidence on the usefulness of MDW in septic conditions.

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来源期刊
Clinical chemistry and laboratory medicine
Clinical chemistry and laboratory medicine 医学-医学实验技术
CiteScore
11.30
自引率
16.20%
发文量
306
审稿时长
3 months
期刊介绍: Clinical Chemistry and Laboratory Medicine (CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor over 3. CCLM is issued monthly, and it is published in print and electronically. CCLM is the official journal of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) and publishes regularly EFLM recommendations and news. CCLM is the official journal of the National Societies from Austria (ÖGLMKC); Belgium (RBSLM); Germany (DGKL); Hungary (MLDT); Ireland (ACBI); Italy (SIBioC); Portugal (SPML); and Slovenia (SZKK); and it is affiliated to AACB (Australia) and SFBC (France). Topics: - clinical biochemistry - clinical genomics and molecular biology - clinical haematology and coagulation - clinical immunology and autoimmunity - clinical microbiology - drug monitoring and analysis - evaluation of diagnostic biomarkers - disease-oriented topics (cardiovascular disease, cancer diagnostics, diabetes) - new reagents, instrumentation and technologies - new methodologies - reference materials and methods - reference values and decision limits - quality and safety in laboratory medicine - translational laboratory medicine - clinical metrology Follow @cclm_degruyter on Twitter!
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