Daniela Ligi, Chiara Della Franca, Michela Pelloso, Alicia Martinez-Iribarren, Alba Leis, Erica Fabbri, Francesca Salvatori, Elena A Sukhacheva, Giorgio Brandi, Giuditta F Schiavano, Ferdinando Mannello
{"title":"大肠杆菌脓毒症单核细胞分布宽度变化的比较分析:来自体内和离体模型的见解。","authors":"Daniela Ligi, Chiara Della Franca, Michela Pelloso, Alicia Martinez-Iribarren, Alba Leis, Erica Fabbri, Francesca Salvatori, Elena A Sukhacheva, Giorgio Brandi, Giuditta F Schiavano, Ferdinando Mannello","doi":"10.1515/cclm-2025-0487","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Monocyte distribution width (MDW) is an early sepsis indicator measuring monocyte heterogeneity during massive infection. We compared MDW changes in <i>Escherichia coli</i> sepsis patients with the effects of living <i>E. coli</i> and lipopolysaccharide in an <i>ex vivo</i> sepsis model. We also investigated the dynamics of monocyte morpho-functional and inflammatory responses in the sepsis model.</p><p><strong>Methods: </strong>Whole blood from healthy participants was <i>in vitro</i> stimulated with live <i>E. coli</i> (10<sup>6</sup>-10<sup>10</sup> CFU/mL) and LPS (0.1-10 μg/mL). Complete blood counts, including MDW, were evaluated at different time-points using DxH 690T Hematology Analyzer (Beckman Coulter). MDW values were compared with those retrospectively obtained from sepsis patients (n=23). May-Grunwald-Giemsa-stained blood smears were analyzed by digital cell morphology (CellaVision DM software). A panel of 27 inflammatory mediators was quantified in plasma (Bio-Plex 200).</p><p><strong>Results: </strong>MDW values were early and significantly increased in a dose- and time-dependent manner by live <i>E. coli</i> and LPS treatments (p<0.01). MDW values were significantly higher in sepsis patients compared to controls and overlapped those observed in the <i>ex vivo</i> model. IL-1β, TNF-α, IL-8, MIP1-α, MIP-1β, Eotaxin, G-CSF, and PDGF-bb were significantly modulated after treatments.</p><p><strong>Conclusions: </strong>Our findings confirm the clinical utility of MDW in sepsis diagnosis and sustain the reliability of the whole blood assay as <i>ex vivo</i> sepsis model. <i>E. coli</i> and LPS directly promote early monocyte morpho-functional modifications, mirrored by high MDW values and pro-inflammatory mediators. These results improve the knowledge on the biological basis of sepsis, providing novel evidence on the usefulness of MDW in septic conditions.</p>","PeriodicalId":10390,"journal":{"name":"Clinical chemistry and laboratory medicine","volume":" ","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative analysis of monocyte distribution width alterations in <i>Escherichia coli</i> sepsis: insights from <i>in vivo</i> and ex vivo models.\",\"authors\":\"Daniela Ligi, Chiara Della Franca, Michela Pelloso, Alicia Martinez-Iribarren, Alba Leis, Erica Fabbri, Francesca Salvatori, Elena A Sukhacheva, Giorgio Brandi, Giuditta F Schiavano, Ferdinando Mannello\",\"doi\":\"10.1515/cclm-2025-0487\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Monocyte distribution width (MDW) is an early sepsis indicator measuring monocyte heterogeneity during massive infection. We compared MDW changes in <i>Escherichia coli</i> sepsis patients with the effects of living <i>E. coli</i> and lipopolysaccharide in an <i>ex vivo</i> sepsis model. We also investigated the dynamics of monocyte morpho-functional and inflammatory responses in the sepsis model.</p><p><strong>Methods: </strong>Whole blood from healthy participants was <i>in vitro</i> stimulated with live <i>E. coli</i> (10<sup>6</sup>-10<sup>10</sup> CFU/mL) and LPS (0.1-10 μg/mL). Complete blood counts, including MDW, were evaluated at different time-points using DxH 690T Hematology Analyzer (Beckman Coulter). MDW values were compared with those retrospectively obtained from sepsis patients (n=23). May-Grunwald-Giemsa-stained blood smears were analyzed by digital cell morphology (CellaVision DM software). A panel of 27 inflammatory mediators was quantified in plasma (Bio-Plex 200).</p><p><strong>Results: </strong>MDW values were early and significantly increased in a dose- and time-dependent manner by live <i>E. coli</i> and LPS treatments (p<0.01). MDW values were significantly higher in sepsis patients compared to controls and overlapped those observed in the <i>ex vivo</i> model. IL-1β, TNF-α, IL-8, MIP1-α, MIP-1β, Eotaxin, G-CSF, and PDGF-bb were significantly modulated after treatments.</p><p><strong>Conclusions: </strong>Our findings confirm the clinical utility of MDW in sepsis diagnosis and sustain the reliability of the whole blood assay as <i>ex vivo</i> sepsis model. <i>E. coli</i> and LPS directly promote early monocyte morpho-functional modifications, mirrored by high MDW values and pro-inflammatory mediators. These results improve the knowledge on the biological basis of sepsis, providing novel evidence on the usefulness of MDW in septic conditions.</p>\",\"PeriodicalId\":10390,\"journal\":{\"name\":\"Clinical chemistry and laboratory medicine\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2025-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical chemistry and laboratory medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1515/cclm-2025-0487\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical chemistry and laboratory medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1515/cclm-2025-0487","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Comparative analysis of monocyte distribution width alterations in Escherichia coli sepsis: insights from in vivo and ex vivo models.
Objectives: Monocyte distribution width (MDW) is an early sepsis indicator measuring monocyte heterogeneity during massive infection. We compared MDW changes in Escherichia coli sepsis patients with the effects of living E. coli and lipopolysaccharide in an ex vivo sepsis model. We also investigated the dynamics of monocyte morpho-functional and inflammatory responses in the sepsis model.
Methods: Whole blood from healthy participants was in vitro stimulated with live E. coli (106-1010 CFU/mL) and LPS (0.1-10 μg/mL). Complete blood counts, including MDW, were evaluated at different time-points using DxH 690T Hematology Analyzer (Beckman Coulter). MDW values were compared with those retrospectively obtained from sepsis patients (n=23). May-Grunwald-Giemsa-stained blood smears were analyzed by digital cell morphology (CellaVision DM software). A panel of 27 inflammatory mediators was quantified in plasma (Bio-Plex 200).
Results: MDW values were early and significantly increased in a dose- and time-dependent manner by live E. coli and LPS treatments (p<0.01). MDW values were significantly higher in sepsis patients compared to controls and overlapped those observed in the ex vivo model. IL-1β, TNF-α, IL-8, MIP1-α, MIP-1β, Eotaxin, G-CSF, and PDGF-bb were significantly modulated after treatments.
Conclusions: Our findings confirm the clinical utility of MDW in sepsis diagnosis and sustain the reliability of the whole blood assay as ex vivo sepsis model. E. coli and LPS directly promote early monocyte morpho-functional modifications, mirrored by high MDW values and pro-inflammatory mediators. These results improve the knowledge on the biological basis of sepsis, providing novel evidence on the usefulness of MDW in septic conditions.
期刊介绍:
Clinical Chemistry and Laboratory Medicine (CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor over 3. CCLM is issued monthly, and it is published in print and electronically.
CCLM is the official journal of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) and publishes regularly EFLM recommendations and news. CCLM is the official journal of the National Societies from Austria (ÖGLMKC); Belgium (RBSLM); Germany (DGKL); Hungary (MLDT); Ireland (ACBI); Italy (SIBioC); Portugal (SPML); and Slovenia (SZKK); and it is affiliated to AACB (Australia) and SFBC (France).
Topics:
- clinical biochemistry
- clinical genomics and molecular biology
- clinical haematology and coagulation
- clinical immunology and autoimmunity
- clinical microbiology
- drug monitoring and analysis
- evaluation of diagnostic biomarkers
- disease-oriented topics (cardiovascular disease, cancer diagnostics, diabetes)
- new reagents, instrumentation and technologies
- new methodologies
- reference materials and methods
- reference values and decision limits
- quality and safety in laboratory medicine
- translational laboratory medicine
- clinical metrology
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