来自孕妇粪便的细胞外囊泡在体外调节T细胞向妊娠支持表型。

IF 3.7 3区 医学 Q2 IMMUNOLOGY
Stefanie Dietz-Ziegler, Samantha Kewitz, Gabriele Kaiser, Jessica Rühle, Alexander Marmé, Alexander Dalpke, Bachar Cheaib, Jan Pauluschke-Fröhlich, Melanie Henes, Ana Velic, Andreas Pich, Anneli Vollert, Martin Schaller, Felix Knab, Trim Lajqi, Christian F. Poets, Christian Gille, Natascha Köstlin-Gille
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引用次数: 0

摘要

怀孕需要对半异体胎儿的免疫耐受,涉及深刻的适应,特别是辅助性T细胞反应。肠道微生物组在健康中起着至关重要的作用,但其对怀孕免疫适应的影响尚不清楚。细菌胞外囊泡(BEVs)是由肠道细菌释放的一种能够穿越肠道屏障并调节免疫反应的物质。在我们的研究中,我们在体外研究了孕妇粪便EVs (fEVs)对Th细胞组成的影响。从保存的粪便样本中纯化发热病毒,对其进行表征,并分析其被免疫细胞摄取的情况。利用体外T细胞培养模型,我们检测了孕妇和非孕妇的feev刺激后的T细胞表型、细胞内细胞因子表达和蛋白质组学变化。我们证明保存的粪便样本中的发热病毒被T细胞迅速吸收并调节其表型。与未怀孕的对照组相比,来自孕妇的feev刺激使Th细胞向有利于妊娠的调节谱转移,增加Th2细胞,减少Th17细胞。该研究首次提供了体外证据,证明粪便来源的ev影响对妊娠的免疫适应,并可能为微生物组靶向策略提供基础,以预防或治疗免疫性妊娠并发症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Extracellular Vesicles Derived From the Feces of Pregnant Women Modulate T Cells Toward a Pregnancy-Supportive Phenotype In Vitro

Extracellular Vesicles Derived From the Feces of Pregnant Women Modulate T Cells Toward a Pregnancy-Supportive Phenotype In Vitro

Pregnancy requires immune tolerance to a semi-allogeneic fetus, involving profound adaptations, particularly in the T helper (Th) cell response. The intestinal microbiome plays a crucial role in health, but its influence on immune adaptation to pregnancy remains unclear. Bacterial extracellular vesicles (BEVs), released by gut bacteria, can cross the intestinal barrier and modulate immune responses. In our study we investigated the effect of fecal EVs (fEVs) from pregnant women on Th cell composition in vitro. fEVs were purified from preserved stool samples, characterized, and their uptake by immune cells was analyzed. Using an in vitro T cell culture model, we examined Th cell phenotypes, intracellular cytokine expression, and proteomic changes after stimulation with fEVs from pregnant and non-pregnant women. We demonstrate that fEVs from preserved stool samples are rapidly taken up by T cells and modulate their phenotype. Stimulation with fEVs from pregnant women shifts Th cells toward a regulatory profile favorable for pregnancy, increasing Th2 cells while reducing Th17 cells compared to fEVs from non-pregnant controls. This study provides the first in vitro evidence that fecal-derived EVs influence immune adaptation to pregnancy and may offer a basis for microbiome-targeted strategies to prevent or treat immunological pregnancy complications.

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来源期刊
CiteScore
8.30
自引率
3.70%
发文量
224
审稿时长
2 months
期刊介绍: The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.
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