MALDI-TOF质谱法检测生物样品中氧化主要磷脂的常规基质和1-芘丁酰肼。

IF 2.7 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Patricia Prabutzki, , , Jürgen Schiller, , and , Kathrin M. Engel*, 
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引用次数: 0

摘要

氧化脂质参与许多与脂质代谢失调和/或低水平慢性炎症相关的广泛疾病。氧化还原失衡导致活性氧增加,导致各种脂质过氧化产物的产生,包括溶脂和截断羰基化合物,特别是羧酸和醛。后者可以很容易地与其他生物分子,如DNA或蛋白质发生反应,从而损害其生物学功能。尽管人们对氧化脂质的作用和功能越来越感兴趣,但它们的分析仍然具有挑战性。这是由于影响其直接分析的几个因素,包括它们的低丰度,结构多样性,瞬态性质以及方法特定因素,如基质辅助激光解吸/电离(MALDI)基质对这种氧化脂质的可检测性的影响。在这里,我们利用不同的MALDI基质(包括2,5-二羟基苯甲酸、4-(二甲氨基)肉桂酸和9-氨基吡啶)评估了不同氧化主要磷脂的可检测性,并以大鼠肝脏提取物作为复杂生物标本的代理。我们还表明,1-芘丁酰肼是天然和氧化磷脂酰胆碱和磷脂酰乙醇胺类脂质MALDI质谱分析的合适基质化合物,因为它既可以作为截断氧化醛类脂的衍生化剂,也可以作为常规(UV)-MALDI基质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MALDI-TOF MS Detection of Oxidized Major Phospholipids in Biological Samples Using Conventional Matrices and 1-Pyrenebutyric Hydrazide

Oxidized lipids are involved in many widespread diseases associated with dysregulated lipid metabolism and/or low-level chronic inflammation. An increase in reactive oxygen species due to redox imbalance leads to the generation of various lipid peroxidation products, including lysolipids and truncated carbonyl compounds, particularly carboxylic acids and aldehydes. The latter can readily react with other biomolecules, such as DNA or proteins, and thereby impair their biological functions. Despite the growing interest in the role and function of oxidized lipids, their analysis remains challenging. This is due to several factors affecting their straightforward analysis, including their low abundance, their structural diversity, and their transient nature as well as method-specific factors such as the impact of matrix-assisted laser desorption/ionization (MALDI) matrices on the detectability of such oxidized lipids. Here, we evaluate the detectability of different oxidized major phospholipids, using different MALDI matrices including 2,5-dihydroxybenzoic acid, 4-(dimethylamino)cinnamic acid, and 9-aminoacridine with rat liver extracts serving as a proxy for complex biological specimen. We also show that 1-pyrenebutyric hydrazide is a suitable matrix compound for the MALDI mass spectrometric analysis of native and oxidized phosphatidylcholine and phosphatidylethanolamine lipids, as it can function both as a derivatization agent for truncated oxidized aldehyde lipids and as a regular (UV)-MALDI matrix.

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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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