Patricia Prabutzki, , , Jürgen Schiller, , and , Kathrin M. Engel*,
{"title":"MALDI-TOF质谱法检测生物样品中氧化主要磷脂的常规基质和1-芘丁酰肼。","authors":"Patricia Prabutzki, , , Jürgen Schiller, , and , Kathrin M. Engel*, ","doi":"10.1021/jasms.5c00196","DOIUrl":null,"url":null,"abstract":"<p >Oxidized lipids are involved in many widespread diseases associated with dysregulated lipid metabolism and/or low-level chronic inflammation. An increase in reactive oxygen species due to redox imbalance leads to the generation of various lipid peroxidation products, including lysolipids and truncated carbonyl compounds, particularly carboxylic acids and aldehydes. The latter can readily react with other biomolecules, such as DNA or proteins, and thereby impair their biological functions. Despite the growing interest in the role and function of oxidized lipids, their analysis remains challenging. This is due to several factors affecting their straightforward analysis, including their low abundance, their structural diversity, and their transient nature as well as method-specific factors such as the impact of matrix-assisted laser desorption/ionization (MALDI) matrices on the detectability of such oxidized lipids. Here, we evaluate the detectability of different oxidized major phospholipids, using different MALDI matrices including 2,5-dihydroxybenzoic acid, 4-(dimethylamino)cinnamic acid, and 9-aminoacridine with rat liver extracts serving as a proxy for complex biological specimen. We also show that 1-pyrenebutyric hydrazide is a suitable matrix compound for the MALDI mass spectrometric analysis of native and oxidized phosphatidylcholine and phosphatidylethanolamine lipids, as it can function both as a derivatization agent for truncated oxidized aldehyde lipids and as a regular (UV)-MALDI matrix.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 10","pages":"2197–2205"},"PeriodicalIF":2.7000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/jasms.5c00196","citationCount":"0","resultStr":"{\"title\":\"MALDI-TOF MS Detection of Oxidized Major Phospholipids in Biological Samples Using Conventional Matrices and 1-Pyrenebutyric Hydrazide\",\"authors\":\"Patricia Prabutzki, , , Jürgen Schiller, , and , Kathrin M. Engel*, \",\"doi\":\"10.1021/jasms.5c00196\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Oxidized lipids are involved in many widespread diseases associated with dysregulated lipid metabolism and/or low-level chronic inflammation. An increase in reactive oxygen species due to redox imbalance leads to the generation of various lipid peroxidation products, including lysolipids and truncated carbonyl compounds, particularly carboxylic acids and aldehydes. The latter can readily react with other biomolecules, such as DNA or proteins, and thereby impair their biological functions. Despite the growing interest in the role and function of oxidized lipids, their analysis remains challenging. This is due to several factors affecting their straightforward analysis, including their low abundance, their structural diversity, and their transient nature as well as method-specific factors such as the impact of matrix-assisted laser desorption/ionization (MALDI) matrices on the detectability of such oxidized lipids. Here, we evaluate the detectability of different oxidized major phospholipids, using different MALDI matrices including 2,5-dihydroxybenzoic acid, 4-(dimethylamino)cinnamic acid, and 9-aminoacridine with rat liver extracts serving as a proxy for complex biological specimen. We also show that 1-pyrenebutyric hydrazide is a suitable matrix compound for the MALDI mass spectrometric analysis of native and oxidized phosphatidylcholine and phosphatidylethanolamine lipids, as it can function both as a derivatization agent for truncated oxidized aldehyde lipids and as a regular (UV)-MALDI matrix.</p>\",\"PeriodicalId\":672,\"journal\":{\"name\":\"Journal of the American Society for Mass Spectrometry\",\"volume\":\"36 10\",\"pages\":\"2197–2205\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-09-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://pubs.acs.org/doi/pdf/10.1021/jasms.5c00196\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the American Society for Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/jasms.5c00196\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Society for Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/jasms.5c00196","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
MALDI-TOF MS Detection of Oxidized Major Phospholipids in Biological Samples Using Conventional Matrices and 1-Pyrenebutyric Hydrazide
Oxidized lipids are involved in many widespread diseases associated with dysregulated lipid metabolism and/or low-level chronic inflammation. An increase in reactive oxygen species due to redox imbalance leads to the generation of various lipid peroxidation products, including lysolipids and truncated carbonyl compounds, particularly carboxylic acids and aldehydes. The latter can readily react with other biomolecules, such as DNA or proteins, and thereby impair their biological functions. Despite the growing interest in the role and function of oxidized lipids, their analysis remains challenging. This is due to several factors affecting their straightforward analysis, including their low abundance, their structural diversity, and their transient nature as well as method-specific factors such as the impact of matrix-assisted laser desorption/ionization (MALDI) matrices on the detectability of such oxidized lipids. Here, we evaluate the detectability of different oxidized major phospholipids, using different MALDI matrices including 2,5-dihydroxybenzoic acid, 4-(dimethylamino)cinnamic acid, and 9-aminoacridine with rat liver extracts serving as a proxy for complex biological specimen. We also show that 1-pyrenebutyric hydrazide is a suitable matrix compound for the MALDI mass spectrometric analysis of native and oxidized phosphatidylcholine and phosphatidylethanolamine lipids, as it can function both as a derivatization agent for truncated oxidized aldehyde lipids and as a regular (UV)-MALDI matrix.
期刊介绍:
The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role.
Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives