基于三（吡唑）的氨基环丙烷羧酸氧化酶模型及其对氧化剂的行为。

IF 2.7 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Biological Inorganic Chemistry Pub Date : 2025-09-12 DOI:10.1007/s00775-025-02125-w

Lars Müller, Charikleia Tzatza, Santina Hoof, A Jalila Simaan, Christian Limberg

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引用次数: 0

摘要

1-氨基环丙烷羧酸氧化酶（ACCO）的一种低分子量类似物[TpMesFeACC]， 1，其中（His 2 Asp）铁（II）部分被水（3-甲酰基吡唑-1-酰基）硼铁（II）单元模拟，其天然底物氨基环丙烷羧酸盐与之配位，已被获取并进行了结构表征。人们发现它与O2反应缓慢，生成生物产物乙烯。为了建立作为ACCO催化循环一部分的中间体的模型，我们在低温下用tBuOOH和mCPBA处理了1，如光谱分析所示，它们分别生成了FeIIIOOtBu和FeIV=O中间体。对其退火行为的研究表明其具有非仿生反应性。

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A tris(pyrazolyl)-based model for the aminocyclopropane carboxylic acid oxidase and its behavior towards oxidants.

A low-molecular-weight analog of the 1-aminocyclopropane carboxylic acid oxidase (ACCO), [Tp^MesFeACC], 1, where the (His₂Asp)iron(II) moiety is mimicked by a hydrotris(3-mesitylpyrazol-1-yl)borato iron(II) unit, to which the natural substrate aminocyclopropane carboxylate is coordinated, has been accessed and structurally characterized. It was found to react slowly with O₂ to yield the biological product ethylene. To create models of the intermediates proposed as part of the catalytic cycle of the ACCO 1 was treated with tBuOOH and mCPBA at low temperatures, which generated the respective Fe^IIIOOtBu and Fe^IV=O intermediates as shown by spectroscopic analysis. Studies on their behavior upon annealing reveal a non-biomimetic reactivity.

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来源期刊

Journal of Biological Inorganic Chemistry 化学-生化与分子生物学

CiteScore

5.90

自引率

3.30%

发文量

审稿时长

3 months

期刊介绍： Biological inorganic chemistry is a growing field of science that embraces the principles of biology and inorganic chemistry and impacts other fields ranging from medicine to the environment. JBIC (Journal of Biological Inorganic Chemistry) seeks to promote this field internationally. The Journal is primarily concerned with advances in understanding the role of metal ions within a biological matrix—be it a protein, DNA/RNA, or a cell, as well as appropriate model studies. Manuscripts describing high-quality original research on the above topics in English are invited for submission to this Journal. The Journal publishes original articles, minireviews, and commentaries on debated issues.