多靶点病原菌废水浓度监测方法的敏感性、通量和成本分析

IF 4.3 Q1 ENVIRONMENTAL SCIENCES
Jingjing Wu, Michael X. Wang, Todd J. Treangen, Katherine Ensor, Loren Hopkins and Lauren B. Stadler*, 
{"title":"多靶点病原菌废水浓度监测方法的敏感性、通量和成本分析","authors":"Jingjing Wu,&nbsp;Michael X. Wang,&nbsp;Todd J. Treangen,&nbsp;Katherine Ensor,&nbsp;Loren Hopkins and Lauren B. Stadler*,&nbsp;","doi":"10.1021/acsestwater.5c00569","DOIUrl":null,"url":null,"abstract":"<p >Wastewater-based epidemiology is an efficient method for monitoring the transmission of diverse pathogens in communities. While various concentration methods are used, most were selected to detect SARS-CoV-2 and other respiratory viruses. Research is needed to guide the method selection for monitoring diverse pathogens in wastewater. In this study, a head-to-head comparison of six different concentration methods was performed, including direct extraction (with and without bead beating), electronegative (HA) filtration, solid concentration, and magnetic bead-based concentration (using Nanotrap particles; with and without bead beating). Methods were assessed for sensitivity, inhibitor removal, recovery rates, and cost, targeting 14 microorganisms including viruses, bacteria, and fungal pathogens. Results showed that the concentration method selection significantly impacts the sensitivity and economic costs of the wastewater monitoring workflow. While no single method was optimal for all targets, combining HA filtration and solid methods in parallel for the same sample is recommended to sensitively detect viruses, bacteria, and fungal pathogens. The magnetic bead-based method can be automated but costs more per sample and is less sensitive for some targets. This study provides data-driven insights to enhance the reliability and cost-effectiveness of wastewater surveillance systems that can support public health responses for a broad range of diseases.</p>","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"5 9","pages":"5531–5541"},"PeriodicalIF":4.3000,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sensitivity, Throughput, and Cost Analysis of Concentration Methods for Multitarget Pathogen Wastewater Monitoring\",\"authors\":\"Jingjing Wu,&nbsp;Michael X. Wang,&nbsp;Todd J. Treangen,&nbsp;Katherine Ensor,&nbsp;Loren Hopkins and Lauren B. Stadler*,&nbsp;\",\"doi\":\"10.1021/acsestwater.5c00569\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Wastewater-based epidemiology is an efficient method for monitoring the transmission of diverse pathogens in communities. While various concentration methods are used, most were selected to detect SARS-CoV-2 and other respiratory viruses. Research is needed to guide the method selection for monitoring diverse pathogens in wastewater. In this study, a head-to-head comparison of six different concentration methods was performed, including direct extraction (with and without bead beating), electronegative (HA) filtration, solid concentration, and magnetic bead-based concentration (using Nanotrap particles; with and without bead beating). Methods were assessed for sensitivity, inhibitor removal, recovery rates, and cost, targeting 14 microorganisms including viruses, bacteria, and fungal pathogens. Results showed that the concentration method selection significantly impacts the sensitivity and economic costs of the wastewater monitoring workflow. While no single method was optimal for all targets, combining HA filtration and solid methods in parallel for the same sample is recommended to sensitively detect viruses, bacteria, and fungal pathogens. The magnetic bead-based method can be automated but costs more per sample and is less sensitive for some targets. This study provides data-driven insights to enhance the reliability and cost-effectiveness of wastewater surveillance systems that can support public health responses for a broad range of diseases.</p>\",\"PeriodicalId\":93847,\"journal\":{\"name\":\"ACS ES&T water\",\"volume\":\"5 9\",\"pages\":\"5531–5541\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2025-09-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS ES&T water\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acsestwater.5c00569\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS ES&T water","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsestwater.5c00569","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

基于废水的流行病学是监测各种病原体在社区传播的有效方法。虽然使用了各种浓度方法,但大多数选择用于检测SARS-CoV-2和其他呼吸道病毒。需要研究指导废水中多种病原菌监测方法的选择。在这项研究中,对六种不同的浓缩方法进行了全面的比较,包括直接提取(有和没有珠粒加热)、电负性(HA)过滤、固体浓缩和磁性珠粒浓缩(使用纳米阱颗粒,有和没有珠粒加热)。对包括病毒、细菌和真菌病原体在内的14种微生物进行了敏感性、抑制剂去除率、回收率和成本评估。结果表明,浓度法的选择对废水监测流程的敏感性和经济成本有显著影响。虽然没有一种方法对所有目标都是最佳的,但建议将HA过滤和固体方法并行用于同一样品,以灵敏地检测病毒,细菌和真菌病原体。基于磁珠的方法可以实现自动化,但每个样品的成本更高,并且对某些目标的灵敏度较低。本研究提供了数据驱动的见解,以提高废水监测系统的可靠性和成本效益,从而支持对广泛疾病的公共卫生反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Sensitivity, Throughput, and Cost Analysis of Concentration Methods for Multitarget Pathogen Wastewater Monitoring

Sensitivity, Throughput, and Cost Analysis of Concentration Methods for Multitarget Pathogen Wastewater Monitoring

Wastewater-based epidemiology is an efficient method for monitoring the transmission of diverse pathogens in communities. While various concentration methods are used, most were selected to detect SARS-CoV-2 and other respiratory viruses. Research is needed to guide the method selection for monitoring diverse pathogens in wastewater. In this study, a head-to-head comparison of six different concentration methods was performed, including direct extraction (with and without bead beating), electronegative (HA) filtration, solid concentration, and magnetic bead-based concentration (using Nanotrap particles; with and without bead beating). Methods were assessed for sensitivity, inhibitor removal, recovery rates, and cost, targeting 14 microorganisms including viruses, bacteria, and fungal pathogens. Results showed that the concentration method selection significantly impacts the sensitivity and economic costs of the wastewater monitoring workflow. While no single method was optimal for all targets, combining HA filtration and solid methods in parallel for the same sample is recommended to sensitively detect viruses, bacteria, and fungal pathogens. The magnetic bead-based method can be automated but costs more per sample and is less sensitive for some targets. This study provides data-driven insights to enhance the reliability and cost-effectiveness of wastewater surveillance systems that can support public health responses for a broad range of diseases.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
5.40
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信