Iratxe Madarieta, Jon Luzuriaga, Jone Salvador-Moya, Beatriz Pardo-Rodríguez, Patricia García-Gallastegui, Nerea García-Urkia, Igor Irastorza, Francisco-Javier Fernandez-San-Argimiro, Verónica Uribe-Etxebarria, Lucía Jiménez-Rojo, Fernando Unda, Beatriz Olalde, Jose Ramon Pineda, Gaskon Ibarretxe
{"title":"利用猪脂肪组织来源的细胞外基质支架和人牙髓干细胞制备用于疾病建模的人源化骨。","authors":"Iratxe Madarieta, Jon Luzuriaga, Jone Salvador-Moya, Beatriz Pardo-Rodríguez, Patricia García-Gallastegui, Nerea García-Urkia, Igor Irastorza, Francisco-Javier Fernandez-San-Argimiro, Verónica Uribe-Etxebarria, Lucía Jiménez-Rojo, Fernando Unda, Beatriz Olalde, Jose Ramon Pineda, Gaskon Ibarretxe","doi":"10.1016/bs.mcb.2025.06.003","DOIUrl":null,"url":null,"abstract":"<p><p>Human Dental Pulp Stem Cells (hDPSCs) represent a remarkable cell source for tissue engineering and regenerative medicine, offering significant potential for use in personalized medicine and autologous therapies. Decellularized extracellular matrix (ECM)-derived biological scaffolds show excellent properties for supporting cell delivery and growth in both in vitro and in vivo applications. These scaffolds provide essential biochemical cues that regulate cellular functions and offer a more accurate representation of the in vivo environment. Porcine decellularized adipose tissue (pDAT) is a very abundant source of ECM, constituting an ideal material for biologic scaffold preparation. The integration of hDPSCs with pDAT-derived ECM enables the patient-specific generation of diverse humanized tissues and their application in personalized drug screening platforms. This chapter details a three dimensional (3D) culture methodology utilizing hDPSCs and pDAT-derived scaffolds to engineer humanized bone tissue.</p>","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"197 ","pages":"59-76"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells.\",\"authors\":\"Iratxe Madarieta, Jon Luzuriaga, Jone Salvador-Moya, Beatriz Pardo-Rodríguez, Patricia García-Gallastegui, Nerea García-Urkia, Igor Irastorza, Francisco-Javier Fernandez-San-Argimiro, Verónica Uribe-Etxebarria, Lucía Jiménez-Rojo, Fernando Unda, Beatriz Olalde, Jose Ramon Pineda, Gaskon Ibarretxe\",\"doi\":\"10.1016/bs.mcb.2025.06.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Human Dental Pulp Stem Cells (hDPSCs) represent a remarkable cell source for tissue engineering and regenerative medicine, offering significant potential for use in personalized medicine and autologous therapies. Decellularized extracellular matrix (ECM)-derived biological scaffolds show excellent properties for supporting cell delivery and growth in both in vitro and in vivo applications. These scaffolds provide essential biochemical cues that regulate cellular functions and offer a more accurate representation of the in vivo environment. Porcine decellularized adipose tissue (pDAT) is a very abundant source of ECM, constituting an ideal material for biologic scaffold preparation. The integration of hDPSCs with pDAT-derived ECM enables the patient-specific generation of diverse humanized tissues and their application in personalized drug screening platforms. This chapter details a three dimensional (3D) culture methodology utilizing hDPSCs and pDAT-derived scaffolds to engineer humanized bone tissue.</p>\",\"PeriodicalId\":18437,\"journal\":{\"name\":\"Methods in cell biology\",\"volume\":\"197 \",\"pages\":\"59-76\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Methods in cell biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/bs.mcb.2025.06.003\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/7/17 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in cell biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/bs.mcb.2025.06.003","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/17 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells.
Human Dental Pulp Stem Cells (hDPSCs) represent a remarkable cell source for tissue engineering and regenerative medicine, offering significant potential for use in personalized medicine and autologous therapies. Decellularized extracellular matrix (ECM)-derived biological scaffolds show excellent properties for supporting cell delivery and growth in both in vitro and in vivo applications. These scaffolds provide essential biochemical cues that regulate cellular functions and offer a more accurate representation of the in vivo environment. Porcine decellularized adipose tissue (pDAT) is a very abundant source of ECM, constituting an ideal material for biologic scaffold preparation. The integration of hDPSCs with pDAT-derived ECM enables the patient-specific generation of diverse humanized tissues and their application in personalized drug screening platforms. This chapter details a three dimensional (3D) culture methodology utilizing hDPSCs and pDAT-derived scaffolds to engineer humanized bone tissue.
期刊介绍：
For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.
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