寡聚化和胞囊偶联是裂变酵母中spa2介导的极化生长聚焦的基础。

IF 3.6 3区 生物学 Q3 CELL BIOLOGY
Journal of cell science Pub Date : 2025-09-01 Epub Date: 2025-09-11 DOI:10.1242/jcs.264071
Liping Ren, Alaina H Willet, Kathleen L Gould
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引用次数: 0

摘要

极化真菌细胞生长需要胞吐的空间限制到离散的皮质结构域。Spa2是一种进化上保守的支架蛋白,由其特有的结构域结构定义,定位于真菌的极化生长位点,并与丝状真菌的菌丝延伸和芽殖酵母交配等形态形成过程有关。裂糖酵母菌是一种被充分研究和阐明极化生长机制的强有力的模式生物。然而,确定Spa2在S. pombe形态发生中的作用一直是难以捉摸的,突出了在定义广泛保守的Spa2功能方面的空白。在此,我们对Spa2在pombe中的靶向机制、相互作用和功能进行了全面的比较分析。我们发现,Spa2中的所有保守结构域都以一种依赖于胞囊和很大程度上不依赖于细胞骨架的方式影响Spa2在极化生长位点的定位。在细胞尖端,稳定的寡聚化Spa2复合物有助于限制生长区,部分是通过为Sec4同源物Ypt2传递Rab gtpase激活蛋白。尽管Spa2蛋白网络具有物种特异性,但我们的研究结果强调了Spa2在增强极化生长的空间焦点方面的进化保守作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oligomerization and exocyst coupling underlie Spa2-mediated focusing of polarized growth in fission yeast.

Polarized cell growth in fungi requires the spatial restriction of exocytosis to discrete cortical domains. Defined by a characteristic domain architecture, the evolutionarily conserved scaffold protein Spa2 localizes to sites of polarized growth in fungi and has been implicated in morphogenic processes including hyphal extension in filamentous fungi and budding yeast mating. Schizosaccharomyces pombe is a well-studied and powerful model organism for elucidating mechanisms of polarized growth. However, identifying a role for Spa2 in S. pombe morphogenesis has been elusive, highlighting a gap in defining a broadly conserved Spa2 function. Here, we undertook a comprehensive and comparative dissection of the targeting mechanisms, interactome and function of Spa2 in S. pombe. We find that all of the conserved domains in Spa2 influence Spa2 localization to sites of polarized growth in an exocyst-dependent and largely cytoskeleton-independent manner. At cell tips, stable complexes of oligomerized Spa2 contribute to constraining the growth zone, in part by delivering the Rab GTPase-activating protein for the Sec4 homolog Ypt2. Despite species-specific wiring of Spa2 protein networks, our results underscore an evolutionarily conserved role for Spa2 in sharpening the spatial focus of polarized growth.

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来源期刊
Journal of cell science
Journal of cell science 生物-细胞生物学
CiteScore
7.30
自引率
2.50%
发文量
393
审稿时长
1.4 months
期刊介绍: Journal of Cell Science publishes cutting-edge science, encompassing all aspects of cell biology.
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