一个漂浮的子宫内膜类器官模型概括了上皮-基质细胞在体外的相互作用。

IF 3.5 3区 生物学 Q3 CELL BIOLOGY
Adriana Bajetto , Alessandra Pattarozzi , Alessandro Corsaro , Beatrice Tremonti , Monica Gatti , Valerio Pisaturo , Luisa Campagnolo , Denise Colia , Elena Pastine , Alessandra Alteri , Mauro Costa , Stefano Thellung , Federica Barbieri , Tullio Florio
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引用次数: 0

摘要

类器官是一种三维结构,其中干细胞、祖细胞和分化细胞自发地组装成类似于原始组织的结构。子宫内膜类器官由组织碎片发育而来,具有遗传稳定性,对激素刺激有反应,具有中空腔、分泌活性和顶基极性。然而,由于缺乏内皮细胞、免疫细胞和间质细胞,它们在模拟黄体中部子宫内膜方面存在一定的局限性,因此提供的上皮-间质相互作用信息有限。我们开发了一个3d模型,使用标准培养基在漂浮的Matrigel™液滴中生成子宫内膜类器官。漂浮的类器官形成腺体样结构,由组织在中心管腔周围的上皮细胞构成,并包含生长在类器官结构内外紧密接触的基质细胞。与传统的子宫内膜类器官类似,浮动类器官保留了子宫内膜和蜕膜基因的表达,通过qRT-PCR对15个基因进行了评估,尽管在大多数情况下,其表达模式类似于在激素分化类器官中观察到的。总之,我们描述了一个简单而快速的模型来生成三维子宫内膜类器官,确保上皮-基质细胞相互作用的持续性,从而促进分化类器官的发展,从而使研究上皮和基质之间的相互调节成为可能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A floating endometrial organoid model recapitulates epithelial-stromal cell interactions in vitro
Organoids are 3D structures in which stem, progenitor and differentiated cells spontaneously assemble into structures resembling the original tissue. Endometrial organoids, developed from tissue fragments, are genetically stable and responsive to hormone stimulation acquiring a hallow lumen, secretory activity and apico-basal polarity. However, they show some limitations in mimicking the midluteal endometrium since they lack endothelial, immune, and stromal cells, thus providing limited information about epithelial-stromal interactions. We developed a 3D-model to generate endometrial organoids in floating Matrigel™ droplets using standard medium. Floating organoids form gland-like structures constituted by epithelial cells organized around a central lumen, and contain stromal cells grown in close contact, either outside or inside the organoid structure. Similarly to conventional endometrial organoids, floating organoids retain the expression of endometrial and decidual genes, assessed by qRT-PCR for a panel of fifteen genes, although with a pattern of expression resembling, in most cases, what observed in hormonally differentiated organoids. In conclusion, we describe a simple and rapid model to generate 3D endometrial organoids, ensuring the persistence of epithelial-stromal cell interaction, which fosters the development of differentiated organoids, thereby enabling the study of the reciprocal modulation between epithelium and stroma.
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来源期刊
Experimental cell research
Experimental cell research 医学-细胞生物学
CiteScore
7.20
自引率
0.00%
发文量
295
审稿时长
30 days
期刊介绍: Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.
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