牛乳铁蛋白对人红细胞膜作用的评价。

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Biomembranes Pub Date : 2025-09-08 DOI:10.1016/j.bbamem.2025.184455

Leilismara Sousa Nogueira , Bárbara Martins Cordeiro , Gilvânia Aparecida Rabelo Cordeiro , Ana Carolina Pacheco Ramos , Douglas Donizetti Raimundo , Grazielle Aparecida Silva Maia , Cristiane de Oliveira Renó , Leonardo Nunes Medeiros , Luciana Estéfani Drumont Carvalho , Vanessa Faria Cortes , Hérica de Lima Santos , Carlos Frederico Leite Fontes , Leandro Augusto Barbosa

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引用次数: 0

摘要

乳铁蛋白（Lf）是一种铁结合糖蛋白，参与多种生物功能，包括铁代谢和免疫反应。牛乳铁蛋白（bLf）因其潜在的治疗应用而备受关注。本研究探讨了bLf对人红细胞膜的影响，重点是Na， k - atp酶（NKA）的调节。用不同浓度的bLf处理全血和红细胞鬼膜，并进行多项测定，以评估酶活性、脂质过氧化和脂质膜组成。首先，我们证明了Fe+2不能增加NKA的活性。另一方面，牛乳铁蛋白显著增加NKA活性，特别是影响α3亚型。此外，bLf治疗导致总磷脂和胆固醇含量增加，表明红细胞膜发生了改变。分子对接分析显示，bLf与α3-NKA之间存在较强的相互作用，但与α1亚型不存在相互作用，支持其潜在的调控作用。此外，bLf降低了Fe3+和硫代巴比妥酸反应物质（TBARS）水平，表明对氧化应激具有保护作用。这些结果表明，bLf可以调节红细胞膜性质，并且可能特异性地激活α3-NKA，从而增强了Fe+3在调节NKA活性中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Evaluation of the effects of bovine lactoferrin on the membrane of human erythrocytes

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Evaluation of the effects of bovine lactoferrin on the membrane of human erythrocytes

Lactoferrin (Lf) is an iron-binding glycoprotein involved in various biological functions, including iron metabolism and immune response. Bovine lactoferrin (bLf) has gained attention due to its potential therapeutic applications. This study investigates the effects of bLf on human erythrocyte membranes, focusing on Na,K-ATPase (NKA) modulation. Whole blood and erythrocyte ghost membranes were treated with different concentrations of bLf, and multiple assays were performed to assess enzyme activity, lipid peroxidation, and lipid membrane composition. First, we demonstrate that Fe⁺² is not able to increase the activity of the NKA. Bovine lactoferrin, on the other hand, significantly increases NKA activity, particularly affecting the α3 isoform. Additionally, bLf treatment led to an increase in total phospholipids and cholesterol content, suggesting modifications in the erythrocyte membrane. Molecular docking analysis revealed a strong interaction between bLf and α3-NKA, but not α1 isoform, supporting a potential regulatory role. Furthermore, bLf reduced Fe³⁺ and Thiobarbituric Acid Reactive Substances (TBARS) levels, indicating a protective effect against oxidative stress. These findings suggest that bLf modulates erythrocyte membrane properties, and could be specific to α3-NKA activation, reinforcing the role of Fe⁺³ in modulating the NKA activity.

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来源期刊

Biochimica et biophysica acta. Biomembranes 生物-生化与分子生物学

CiteScore

8.20

自引率

5.90%

发文量

175

审稿时长

2.3 months

期刊介绍： BBA Biomembranes has its main focus on membrane structure, function and biomolecular organization, membrane proteins, receptors, channels and anchors, fluidity and composition, model membranes and liposomes, membrane surface studies and ligand interactions, transport studies, and membrane dynamics.