质膜标记效率,内化和分配功能化荧光脂作为脂质结构的功能。

IF 3.1 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Erdinc Sezgin
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引用次数: 0

摘要

标记质膜用于高级成像仍然是一个重大挑战。对于延时活细胞成像,探针内化和光漂白是影响大多数膜特异性染料的主要限制。在固定或渗透细胞中,许多膜探针在固定后失去信号或不能保持在质膜上。因此,改进探针在空间生物学中的应用是迫切需要的。在这项研究中,我们系统地比较了一系列定制合成的和市售的脂质探针在活细胞、固定细胞和渗透细胞中标记质膜的效率。我们确定了一种优越的探针,由于其脂质结构,它优于其他探针。这种比较提供了使用先进成像技术可视化质膜的理想脂质探针的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure

Plasma membrane labelling efficiency, internalization and partitioning of functionalized fluorescent lipids as a function of lipid structure

Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membrane-specific dyes. In fixed or permeabilized cells, many membrane probes either lose signal after fixation or fail to remain localized to the plasma membrane. Thus, improved probes are critically needed for applications in spatial biology. In this study, we systematically compared a range of custom-synthesized and commercially available lipid-based probes for their efficiency in labeling the plasma membrane in live, fixed, and permeabilized cells. We identified a superior probe, which outperformed others due to its lipid structure. This comparison provides insights into ideal lipid probes for visualizing the plasma membrane using advanced imaging techniques.

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来源期刊
CiteScore
6.10
自引率
0.00%
发文量
128
审稿时长
10 weeks
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