Jim Mansoor, Eva Borras, Emily Mae Wong, Liliana Rodriguez, Amanda Silveira, Catterina Ferreccio, Cristina E Davis, Craig Steinmaus, Edward Schelegle
{"title":"使用呼气冷凝物分析评估早期砷暴露的肺氧化应激和炎症状态。","authors":"Jim Mansoor, Eva Borras, Emily Mae Wong, Liliana Rodriguez, Amanda Silveira, Catterina Ferreccio, Cristina E Davis, Craig Steinmaus, Edward Schelegle","doi":"10.1088/1752-7163/ae0510","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Millions of people worldwide are exposed to environmental arsenic in drinking water, resulting in both malignant and nonmalignant diseases. Interestingly, early life exposure by itself is sufficient to produce higher incidences of these diseases later in life. Based on the delayed onset of disease, we hypothesized that early life arsenic exposure would also induce long-term alterations in the metabolic profile. The objective of this study was to examine metabolomic biomarkers in exhaled breath condensate (EBC) of individuals exposed to arsenic in drinking water early in life, but not later. 
Subjects and Methods: One hundred and fifty subjects (75 males and 75 females) were initially recruited from Antofagasta, Chile, some of whom were exposed to high water arsenic levels (≥870 µg/L; HighAE group), and others, low water arsenic levels (≤110 µg/L; LowAE group) early in life (1958-1970). Exhaled breath condensate samples were collected for targeted and untargeted metabolomic biomarker analysis.
Results: The results showed significantly shorter individuals and reduced pulmonary functions (forced vital capacity, FVC and forced expiratory volume in 1 sec, FEV1) in both males and females in the high-arsenic groups. Males exposed to high arsenic levels also had reduced red blood cell concentrations, as well as higher concentrations of the oxidative stress metabolites 8-OH-2dG and 8-iso-PGF2a. Females in the high-arsenic group showed reductions in 8-OH-2dG. Untargeted analysis revealed metabolomic markers that differentiated the HighAE group from the LowAE group, with a subgroup of markers whose concentrations were proportional to the level of arsenic exposure. 
Conclusion: Targeted and untargeted analyses of EBC using LC-MS indicated that adults exposed to high arsenic levels in drinking water in utero and during early childhood retained a modified metabolic profile 47 years after the end of exposure.
.</p>","PeriodicalId":15306,"journal":{"name":"Journal of breath research","volume":" ","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of lung oxidative stress and inflammatory state using exhaled breath condensate analysis in early-life arsenic exposure.\",\"authors\":\"Jim Mansoor, Eva Borras, Emily Mae Wong, Liliana Rodriguez, Amanda Silveira, Catterina Ferreccio, Cristina E Davis, Craig Steinmaus, Edward Schelegle\",\"doi\":\"10.1088/1752-7163/ae0510\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Millions of people worldwide are exposed to environmental arsenic in drinking water, resulting in both malignant and nonmalignant diseases. Interestingly, early life exposure by itself is sufficient to produce higher incidences of these diseases later in life. Based on the delayed onset of disease, we hypothesized that early life arsenic exposure would also induce long-term alterations in the metabolic profile. The objective of this study was to examine metabolomic biomarkers in exhaled breath condensate (EBC) of individuals exposed to arsenic in drinking water early in life, but not later. 
Subjects and Methods: One hundred and fifty subjects (75 males and 75 females) were initially recruited from Antofagasta, Chile, some of whom were exposed to high water arsenic levels (≥870 µg/L; HighAE group), and others, low water arsenic levels (≤110 µg/L; LowAE group) early in life (1958-1970). Exhaled breath condensate samples were collected for targeted and untargeted metabolomic biomarker analysis.
Results: The results showed significantly shorter individuals and reduced pulmonary functions (forced vital capacity, FVC and forced expiratory volume in 1 sec, FEV1) in both males and females in the high-arsenic groups. Males exposed to high arsenic levels also had reduced red blood cell concentrations, as well as higher concentrations of the oxidative stress metabolites 8-OH-2dG and 8-iso-PGF2a. Females in the high-arsenic group showed reductions in 8-OH-2dG. Untargeted analysis revealed metabolomic markers that differentiated the HighAE group from the LowAE group, with a subgroup of markers whose concentrations were proportional to the level of arsenic exposure. 
Conclusion: Targeted and untargeted analyses of EBC using LC-MS indicated that adults exposed to high arsenic levels in drinking water in utero and during early childhood retained a modified metabolic profile 47 years after the end of exposure.
.</p>\",\"PeriodicalId\":15306,\"journal\":{\"name\":\"Journal of breath research\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2025-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of breath research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1088/1752-7163/ae0510\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of breath research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1088/1752-7163/ae0510","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Evaluation of lung oxidative stress and inflammatory state using exhaled breath condensate analysis in early-life arsenic exposure.
Introduction: Millions of people worldwide are exposed to environmental arsenic in drinking water, resulting in both malignant and nonmalignant diseases. Interestingly, early life exposure by itself is sufficient to produce higher incidences of these diseases later in life. Based on the delayed onset of disease, we hypothesized that early life arsenic exposure would also induce long-term alterations in the metabolic profile. The objective of this study was to examine metabolomic biomarkers in exhaled breath condensate (EBC) of individuals exposed to arsenic in drinking water early in life, but not later.
Subjects and Methods: One hundred and fifty subjects (75 males and 75 females) were initially recruited from Antofagasta, Chile, some of whom were exposed to high water arsenic levels (≥870 µg/L; HighAE group), and others, low water arsenic levels (≤110 µg/L; LowAE group) early in life (1958-1970). Exhaled breath condensate samples were collected for targeted and untargeted metabolomic biomarker analysis.
Results: The results showed significantly shorter individuals and reduced pulmonary functions (forced vital capacity, FVC and forced expiratory volume in 1 sec, FEV1) in both males and females in the high-arsenic groups. Males exposed to high arsenic levels also had reduced red blood cell concentrations, as well as higher concentrations of the oxidative stress metabolites 8-OH-2dG and 8-iso-PGF2a. Females in the high-arsenic group showed reductions in 8-OH-2dG. Untargeted analysis revealed metabolomic markers that differentiated the HighAE group from the LowAE group, with a subgroup of markers whose concentrations were proportional to the level of arsenic exposure.
Conclusion: Targeted and untargeted analyses of EBC using LC-MS indicated that adults exposed to high arsenic levels in drinking water in utero and during early childhood retained a modified metabolic profile 47 years after the end of exposure.
.
期刊介绍：
Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics.
Typical areas of interest include:
Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research.
Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments.
Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway.
Cellular and molecular level in vitro studies.
Clinical, pharmacological and forensic applications.
Mathematical, statistical and graphical data interpretation.
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