揭示CCL3在鼻咽癌中的关键作用:生物信息学和免疫组织化学见解。

IF 3 Q3 PATHOLOGY
Xiaopeng Guo, Zhen Sun, Ya Liang, Aoshuang Chang, Junjun Ling, Houyu Zhao, Xianlu Zhuo
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引用次数: 0

摘要

背景:C-C基序趋化因子配体3 (C-C motif chemokine ligand 3, CCL3)是一种重要的趋化因子,在免疫微环境中起着基础性作用,与多种癌症的发生发展密切相关。尽管CCL3在鼻咽癌中的作用很重要,但目前关于CCL3在鼻咽癌中的表达和功能的研究还很有限。因此,本研究试图通过生物信息学分析和实验来检测CCL3在鼻咽癌中的表达,并评估其临床意义。方法:采用生物信息学方法评价CCL3在鼻咽癌组织中的表达及功能。随后,使用基于组织微阵列的免疫组织化学技术检测了NPC队列中CCL3的蛋白表达。研究CCL3表达与临床特征的关系。结果:通过生物信息学分析,共鉴定出20个ccl3相关基因和14个可能的靶基因,其中许多基因在趋化因子信号通路和肿瘤信号通路的转录失调等通路中起着至关重要的作用。发现CCL3与耐药和各种免疫细胞浸润有关。在鼻咽癌中,CCL3的表达明显高于正常对照组,且CCL3的高表达与颈部淋巴结转移、肿瘤复发、临床分期较晚、预后较差相关。结论:CCL3可能是鼻咽癌发生发展的关键基因。它有潜力作为鼻咽癌的诊断生物标志物和治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unraveling the crucial role of CCL3 in nasopharyngeal carcinoma: bioinformatics and immunohistochemical insights.

Background: C-C motif chemokine ligand 3 (CCL3) is a crucial chemokine that plays a fundamental role in the immune microenvironment and is closely linked to the development of various cancers. Despite its importance, there is limited research regarding the expression and function of CCL3 in nasopharyngeal carcinoma (NPC). Therefore, this study seeks to examine the expression of CCL3 and assess its clinical significance in NPC using bioinformatics analysis and experiments.

Methods: The bioinformatics approach was employed to assess the expression and function of CCL3 in NPC. Subsequently, protein expression of CCL3 was detected in an NPC cohort using immunohistochemistry based on a tissue microarray. The relationship between CCL3 expression and clinical features was then investigated.

Results: A total of 20 CCL3-related genes and 14 possible target genes were identified through bioinformatics analysis, many of which play crucial roles in pathways such as chemokine signaling pathway and transcriptional misregulation in cancer signaling pathways. CCL3 was found to be associated with drug resistance and various immune cell infiltrations. In NPC, CCL3 expression was significantly higher than normal controls, and high expression of CCL3 correlated with cervical lymph node metastasis, tumor recurrence, advanced clinical stage, and poor prognosis.

Conclusions: CCL3 may be a key gene in the initiation and progression of NPC. It has the potential to serve as both a diagnostic biomarker and a therapeutic target for NPC.

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来源期刊
CiteScore
5.00
自引率
4.20%
发文量
45
审稿时长
14 weeks
期刊介绍: The Journal of Pathology and Translational Medicine is an open venue for the rapid publication of major achievements in various fields of pathology, cytopathology, and biomedical and translational research. The Journal aims to share new insights into the molecular and cellular mechanisms of human diseases and to report major advances in both experimental and clinical medicine, with a particular emphasis on translational research. The investigations of human cells and tissues using high-dimensional biology techniques such as genomics and proteomics will be given a high priority. Articles on stem cell biology are also welcome. The categories of manuscript include original articles, review and perspective articles, case studies, brief case reports, and letters to the editor.
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