探讨基于姜黄素的水凝胶珠对弗氏完全佐剂诱导的类风湿关节炎大鼠的有益作用。

IF 3.7 2区 生物学 Q3 CELL BIOLOGY
Katarina Djordjevic, Bozidar Pindovic, Katarina Mihajlovic, Igor Ilic, Jelena Terzic, Andjela Milojevic-Samanovic, Zorka Stanic, Katarina Postolovic, Natalia Ekkert, Vladimir Reshetnikov, Vladimir Zivkovic, Vladimir Jakovljevic, Tamara Nikolic Turnic
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引用次数: 0

摘要

本研究的目的是研究姜黄素在治疗和预防条件下的潜在抗氧化活性及其作为传统药物甲氨蝶呤辅助治疗类风湿性关节炎(RA)的潜在作用。选取6周龄、体重200 ~ 250 g的Wistar白化雌性大鼠104只,随机分为8组,每组13只。CTRL:负控制,2。CUR:阳性对照1(姜黄素200 mg/kg,每周3次,每次4周);甲氨蝶呤:阳性对照2(甲氨蝶呤0.75 mg/kg /次,每周2次,连用4周);RA:阳性对照3(诱导RA), 5。5 . RA+pCUR:诱导RA的大鼠+预防给药姜黄素(姜黄素200 mg/kg,每周3次,每4周诱导RA);RA+tCUR:诱导RA+姜黄素治疗大鼠;RA + MTX + pCUR;7 . RA诱导大鼠+姜黄素+甲氨蝶呤0.75 mg/kg i.p. 2次,连续4周;RA+MTX+tCUR:诱导RA大鼠+姜黄素+甲氨蝶呤0.75 mg/kg,每周2次,连续4周。姜黄素(200mg /kg,每周口服3次,连用4周)的潜在治疗效果与RA诱导在同一天开始;预防方案,在RA诱导开始前4周给予姜黄素,剂量为200mg /kg,每周口服3次。通过在大鼠尾部皮下注射0.1 ml完全弗氏佐剂(CFA)触发类风湿关节炎。免疫后28天处死大鼠。在实验期间,我们收集了关节炎评分、x线检查和血液样本收集的数据,用于对前氧化和抗氧化参数(超氧化物阴离子自由基、过氧化氢、一氧化氮、脂质过氧化指数、超氧化物歧化酶、过氧化氢酶和还原性谷胱甘肽)进行特定的生化分析。我们的实验研究证实,姜黄素的治疗性管理降低了疾病活动,并显着增加了抗氧化酶的活性和减少的促氧化剂。此外,预防性给药,特别是与甲氨蝶呤联合使用,通过限制氧化应激的发生和发展,提供了更好的保护。这些发现支持姜黄素可以有效抑制炎症反应,并通过改变氧化还原状态减轻症状的说法。未来需要对姜黄物质的作用进行大型随机对照试验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploring the beneficial effects of curcumin-based hydrogel beads in rats with rheumatoid arthritis induced by Freund's complete adjuvant.

The aim of this study was to examine the potential antioxidant activity of curcumin in therapeutic and preventive condition and its potential role as adjuvant to conventional drug methotrexate in treatment of rheumatoid arthritis (RA). The study included 104 female Wistar albino rats, 6 weeks old, body weight of 200-250 g, which were divided into 8 groups (n=13 in each group): 1. CTRL: negative control, 2. CUR: positive control 1 (curcumin 200 mg/kg three times a week for 4 weeks per os), 3. MTX: positive control 2 (methotrexate 0,75 mg/kg i.p. two times a week for 4 weeks), 4. RA: positive control 3 (induced RA), 5. RA+pCUR: rats with induced RA + preventive administration of curcumin (curcumin 200 mg/kg three times a week for 4 weeks per os before the induction of RA), 6. RA+tCUR: rats with induced RA + curcumin therapy, 7. RA+MTX+pCUR; rats with induced RA + preventive administration of curcumin + methotrexate 0.75 mg/kg i.p. twice weekly for 4 weeks, 8. RA+MTX+tCUR: rats with induced RA + therapeutic administration of curcumin + methotrexate 0.75 mg/kg i.p. twice weekly for 4 weeks. Potential therapeutic effect of curcumin (200 mg/kg three times a week for 4 weeks orally) and RA induction were initiated on the same day; for preventive protocol, curcumin was administered 4 weeks before the start of RA induction at a dose of 200 mg/kg orally three times a week. Rheumatoid arthritis was triggered by administering 0.1 ml of Complete Freund's Adjuvant (CFA) subcutaneously at the base of the rat tail. Rats were sacrificed 28 days after immunization. During the experimental period, we collected data for arthritis score, radiography testing, and blood sample collecting for specific biochemical analysis for prooxidative and antioxidative parameters (superoxide anion radical, hydrogen peroxide, nitric oxide, index of lipid peroxidation, superoxide dismutase, catalase, and reduced glutathione). Our experimental study confirmed that the therapeutic administration of curcumin reduced disease activity and significantly increased the activities of antioxidative enzymes and reduced prooxidants. Also, preventive administration, especially in combination with methotrexate, offers superior protection by limiting the onset and development of oxidative stress. These findings support the claim that curcumin as turmeric can effectively inhibit inflammatory reactions and reduces symptoms through changing the redox status. Future large randomized controlled trials on the effects of turmeric substance are needed.

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来源期刊
Molecular and Cellular Biochemistry
Molecular and Cellular Biochemistry 生物-细胞生物学
CiteScore
8.30
自引率
2.30%
发文量
293
审稿时长
1.7 months
期刊介绍: Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease publishes original research papers and short communications in all areas of the biochemical sciences, emphasizing novel findings relevant to the biochemical basis of cellular function and disease processes, as well as the mechanics of action of hormones and chemical agents. Coverage includes membrane transport, receptor mechanism, immune response, secretory processes, and cytoskeletal function, as well as biochemical structure-function relationships in the cell. In addition to the reports of original research, the journal publishes state of the art reviews. Specific subjects covered by Molecular and Cellular Biochemistry include cellular metabolism, cellular pathophysiology, enzymology, ion transport, lipid biochemistry, membrane biochemistry, molecular biology, nuclear structure and function, and protein chemistry.
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