Yuan Zhang, Yuyang Peng, Chengcheng Wang, Hong Liang, Song Li, Hui Yang
{"title":"发现催乳素瘤中与性别差异相关的中枢基因。","authors":"Yuan Zhang, Yuyang Peng, Chengcheng Wang, Hong Liang, Song Li, Hui Yang","doi":"10.1177/19450265251375945","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Background:</i></b> Male patients with prolactinomas exhibit greater invasiveness, resistance to dopamine agonists, making treatment more challenging. This study aims to explore the potential different genes contributing to sex disparities in prolactinomas. <b><i>Materials and Methods:</i></b> Weighted gene co-expression network analysis and differential expressed genes analysis were performed to identify sex-related hub genes. In addition, bioinformatics analyses were conducted to understand gene localization on chromosomes, gene regulatory networks, signaling pathways, and their relationship with immune function, which was verified in 21 human prolactinoma samples. <b><i>Results:</i></b> A total of 21 sex-related hub genes were identified. The hub genes in males included nine Y chromosome genes and six autosomal genes, while females had six specific genes. Further predictions using the NetworkAnalyst online tool suggested that transcription factors (REST, androgen receptor) and microRNAs (miR-27a-3p, miR-146a-5p) may be involved in regulating the above sex-related hub genes. CIBERSORT analysis revealed that prolactinomas in males showed significant infiltration of resting dendritic cells and naive CD4<sup>+</sup> T cells. Correlation analysis between sex-related hub genes and immune checkpoint genes indicated that male hub genes were positively correlated with <i>CD47</i> and <i>CEACAM1</i>, while showing a strong negative correlation with <i>CD28</i>, <i>TNFSF14</i>, and <i>CD226</i>. Finally, similar changes of gene expression in our surgical prolactinoma samples were confirmed by RT-qPCR. <b><i>Conclusions:</i></b> In prolactinomas, the male hub genes and female hub genes are identified by our bioinformatics analysis. Our findings suggest that <i>KDM5D</i>, <i>PDCD1</i>, <i>ELOA3BP</i>, <i>XRRA1,</i> and <i>SIGLEC12</i> serve as potential biomarkers for male prolactinomas, while <i>SOX3</i>, <i>DMGDH</i>, and <i>NPAS1</i> may serve as potential biomarkers for female prolactinoma, providing a theoretical basis for targeted therapy.</p>","PeriodicalId":12603,"journal":{"name":"Genetic testing and molecular biomarkers","volume":" ","pages":"241-254"},"PeriodicalIF":1.0000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identifying Hub Genes Associated with Sex Disparities in Prolactinomas.\",\"authors\":\"Yuan Zhang, Yuyang Peng, Chengcheng Wang, Hong Liang, Song Li, Hui Yang\",\"doi\":\"10.1177/19450265251375945\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b><i>Background:</i></b> Male patients with prolactinomas exhibit greater invasiveness, resistance to dopamine agonists, making treatment more challenging. This study aims to explore the potential different genes contributing to sex disparities in prolactinomas. <b><i>Materials and Methods:</i></b> Weighted gene co-expression network analysis and differential expressed genes analysis were performed to identify sex-related hub genes. In addition, bioinformatics analyses were conducted to understand gene localization on chromosomes, gene regulatory networks, signaling pathways, and their relationship with immune function, which was verified in 21 human prolactinoma samples. <b><i>Results:</i></b> A total of 21 sex-related hub genes were identified. The hub genes in males included nine Y chromosome genes and six autosomal genes, while females had six specific genes. Further predictions using the NetworkAnalyst online tool suggested that transcription factors (REST, androgen receptor) and microRNAs (miR-27a-3p, miR-146a-5p) may be involved in regulating the above sex-related hub genes. CIBERSORT analysis revealed that prolactinomas in males showed significant infiltration of resting dendritic cells and naive CD4<sup>+</sup> T cells. Correlation analysis between sex-related hub genes and immune checkpoint genes indicated that male hub genes were positively correlated with <i>CD47</i> and <i>CEACAM1</i>, while showing a strong negative correlation with <i>CD28</i>, <i>TNFSF14</i>, and <i>CD226</i>. Finally, similar changes of gene expression in our surgical prolactinoma samples were confirmed by RT-qPCR. <b><i>Conclusions:</i></b> In prolactinomas, the male hub genes and female hub genes are identified by our bioinformatics analysis. Our findings suggest that <i>KDM5D</i>, <i>PDCD1</i>, <i>ELOA3BP</i>, <i>XRRA1,</i> and <i>SIGLEC12</i> serve as potential biomarkers for male prolactinomas, while <i>SOX3</i>, <i>DMGDH</i>, and <i>NPAS1</i> may serve as potential biomarkers for female prolactinoma, providing a theoretical basis for targeted therapy.</p>\",\"PeriodicalId\":12603,\"journal\":{\"name\":\"Genetic testing and molecular biomarkers\",\"volume\":\" \",\"pages\":\"241-254\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic testing and molecular biomarkers\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1177/19450265251375945\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/9/4 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic testing and molecular biomarkers","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1177/19450265251375945","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/9/4 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Identifying Hub Genes Associated with Sex Disparities in Prolactinomas.
Background: Male patients with prolactinomas exhibit greater invasiveness, resistance to dopamine agonists, making treatment more challenging. This study aims to explore the potential different genes contributing to sex disparities in prolactinomas. Materials and Methods: Weighted gene co-expression network analysis and differential expressed genes analysis were performed to identify sex-related hub genes. In addition, bioinformatics analyses were conducted to understand gene localization on chromosomes, gene regulatory networks, signaling pathways, and their relationship with immune function, which was verified in 21 human prolactinoma samples. Results: A total of 21 sex-related hub genes were identified. The hub genes in males included nine Y chromosome genes and six autosomal genes, while females had six specific genes. Further predictions using the NetworkAnalyst online tool suggested that transcription factors (REST, androgen receptor) and microRNAs (miR-27a-3p, miR-146a-5p) may be involved in regulating the above sex-related hub genes. CIBERSORT analysis revealed that prolactinomas in males showed significant infiltration of resting dendritic cells and naive CD4+ T cells. Correlation analysis between sex-related hub genes and immune checkpoint genes indicated that male hub genes were positively correlated with CD47 and CEACAM1, while showing a strong negative correlation with CD28, TNFSF14, and CD226. Finally, similar changes of gene expression in our surgical prolactinoma samples were confirmed by RT-qPCR. Conclusions: In prolactinomas, the male hub genes and female hub genes are identified by our bioinformatics analysis. Our findings suggest that KDM5D, PDCD1, ELOA3BP, XRRA1, and SIGLEC12 serve as potential biomarkers for male prolactinomas, while SOX3, DMGDH, and NPAS1 may serve as potential biomarkers for female prolactinoma, providing a theoretical basis for targeted therapy.
期刊介绍:
Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results.
Genetic Testing and Molecular Biomarkers coverage includes:
-Diagnosis across the life span-
Risk assessment-
Carrier detection in individuals, couples, and populations-
Novel methods and new instrumentation for genetic testing-
Results of molecular, biochemical, and cytogenetic testing-
Genetic counseling