盐增强MALDI TIMS对中性脂的高特异性和灵敏度成像。

IF 2.7 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Kameron R. Molloy, , , Martin Dufresne, , , Madeline E. Colley, , , Lukasz G. Migas, , , Raf Van de Plas, , and , Jeffrey M. Spraggins*, 
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引用次数: 0

摘要

中性脂对各种细胞过程和疾病病理至关重要。然而,由于电离效率低,以及难以区分众多同分异构体和等压物质之间细微的结构差异,利用基质辅助激光解吸/电离成像质谱(MALDI IMS)对它们进行表征仍然具有挑战性。在这项研究中,我们通过将等渗金属阳离子洗涤纳入我们的MALDI IMS样品制备工作流程来增强中性脂质检测。由此产生的盐加合物改善了中性脂质等压条和同分异构体的分离,采用了捕获离子迁移谱法(TIMS)。该方法增加了跨多种器官类型的中性脂质IMS实验的敏感性和特异性,包括小鼠脑、兔肾上腺、人结肠和人肾脏。比较分析显示,最有效的盐洗是组织依赖性的。然而,Na+碳酸盐缓冲溶液(CBS)洗涤显示出最大的中性脂检测总体增加。这些发现为绘制跨多种组织和疾病状态的中性脂提供了一个强大的框架,并允许在复杂的生物组织中详细表征中性脂异构体和等柱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High-Specificity and Sensitivity Imaging of Neutral Lipids Using Salt-Enhanced MALDI TIMS

Neutral lipids are vital to various cellular processes and disease pathologies. However, their characterization by matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) remains challenging due to poor ionization efficiency and difficulties distinguishing subtle structural differences among numerous isomeric and isobaric species. In this study, we enhanced neutral lipid detection by incorporating isotonic metal–cation washes into our MALDI IMS sample preparation workflow. Resulting salt adducts improved neutral lipid isobar and isomer separation by using trapped ion mobility spectrometry (TIMS). This approach increased both sensitivity and specificity for neutral lipid IMS experiments across multiple organ types, including murine brain, rabbit adrenal gland, human colon, and human kidney. Comparative analyses revealed that the most effective salt wash was tissue-dependent. However, the Na+ carbonate buffer solution (CBS) wash showed the greatest overall increase in neutral lipid detection. These findings provide a robust framework for mapping neutral lipids across multiple tissues and disease states and allow for the detailed characterization of neutral lipid isomers and isobars in complex biological tissues.

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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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