β-内酰胺酶底物特异性和外膜通透性对β-内酰胺敏感菌的抗生素庇护作用的贡献。

IF 3.1 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Montserrat Mora-Ochomogo, Mitchell A Jeffs, Josephine L Liu, Christopher T Lohans
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引用次数: 0

摘要

β-内酰胺类抗生素的使用受到产生β-内酰胺酶的耐药细菌的威胁。这些酶不仅能保护产生它们的细菌,还能在同样的环境中保护其他易受感染的细菌。虽然这种现象具有临床意义,但许多导致β-内酰胺酶介导的抗生素庇护的因素尚未得到充分研究。我们报道了在β-内酰胺酶产生菌和β-内酰胺类抗生素存在的情况下,直接监测β-内酰胺敏感菌存活的发光试验的发展。该方法提供了一种快速、可扩展的方法来量化混合微生物群体中的抗生素庇护。我们应用这个实验来研究几个因素对遮蔽的贡献,包括β-内酰胺的种类,β-内酰胺酶的底物特异性,以及β-内酰胺酶产生细菌的细胞壁通透性。我们的研究结果表明,发生的庇护程度不仅取决于β-内酰胺和β-内酰胺酶的特定组合,而且还受到β-内酰胺酶接近其β-内酰胺底物的能力的极大影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Contributions of β-lactamase substrate specificity and outer membrane permeability to the antibiotic sheltering of β-lactam-susceptible bacteria.

The use of β-lactam antibiotics is threatened by antibiotic resistant bacteria that produce β-lactamases. These enzymes not only protect the bacteria that produce them but also shelter other bacteria in the same environment that would otherwise be susceptible. While this phenomenon is of clinical significance, many of the factors that contribute to β-lactamase-mediated antibiotic sheltering have not been well-studied. We report the development of a luminescence assay to directly monitor the survival of β-lactam-susceptible bacteria in the presence of β-lactamase-producing bacteria and β-lactam antibiotics. This method provides a rapid and scalable means of quantifying antibiotic sheltering in mixed microbial populations. We applied this assay to investigate the contributions of several factors to sheltering, including the class of β-lactam, the substrate specificity of the β-lactamase, and the cell wall permeability of the β-lactamase-producing bacterium. Our results show that the extent of sheltering that occurs not only depends on the particular combination of β-lactam and β-lactamase, but is also greatly impacted by the ability of a β-lactamase to access its β-lactam substrates.

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来源期刊
CiteScore
6.10
自引率
0.00%
发文量
128
审稿时长
10 weeks
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