子宫内膜和肝脏类器官免疫染色的简化方法。

IF 2.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Islam M Saadeldin, Eman Alshehri, Maha AlThubyani, Falah Almohanna, Goran Matic, Hala A Ahmed, Tanveer Ahmad Mir, Abdullah M Assiri
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引用次数: 0

摘要

来源于子宫内膜和肝脏等组织的类器官代表了在三维框架中研究细胞和分子过程的关键模型。这些系统可以通过各种方法(包括免疫染色)对器官发育和疾病病理进行详细的研究,以精确地可视化和定位特定蛋白质。这种简化的方法概述了使用transwell插入物分离、培养和免疫染色个体类器官的系统方案。这种简化的方法优化了固定、渗透和单抗体或双抗体染色的程序,确保了稳健和可重复的结果。我们的方法允许在不干扰矩阵穹顶的3D结构的情况下选择一个单一的类器官。通过标准化这些关键步骤,研究人员可以有效地利用类器官培养来推进我们对复杂生物学机制的理解,并探索针对影响子宫内膜和肝脏疾病的治疗干预措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A simplified method for immunostaining single endometrial and liver organoids.

A simplified method for immunostaining single endometrial and liver organoids.

A simplified method for immunostaining single endometrial and liver organoids.

A simplified method for immunostaining single endometrial and liver organoids.

Organoids derived from tissues such as the endometrium and liver represent crucial models for investigating cellular and molecular processes in a three-dimensional framework. These systems enable detailed studies of organ development and disease pathology through various methods, including immunostaining, for precise visualization and localization of specific proteins. This simplified method outlines a systematic protocol for isolating, culturing, and immunostaining individual organoids using transwell inserts. This streamlined method optimizes procedures for fixation, permeabilization, and single or double antibody staining, ensuring robust and reproducible outcomes. Our method allows selecting a single organoid in a temporal manner without disturbing the 3D structure of Matrigel domes. By standardizing these critical steps, researchers can efficiently utilize organoid cultures to advance our understanding of complex biological mechanisms and explore therapeutic interventions targeting diseases affecting the endometrium and liver.

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