山羊环状病毒新毒株的鉴定：中国首次检测和基因组分析。

IF 3.1 2区农林科学 Q1 VETERINARY SCIENCES

Irish Veterinary Journal Pub Date : 2025-08-27 DOI:10.1186/s13620-025-00305-3

Kegu Ji'e, Falong Yang, Yang Su, Keha-Mo Abi

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引用次数: 0

摘要

利用病毒元基因组学技术，从山羊腹泻粪便样本中鉴定出一种基因组长度为28,457 nt的新型环状病毒GToV/SWUN/SC，该毒株与羚羊环状病毒（AToV）核苷酸同源性为96.73 ~ 96.79%，与GToV/SZ毒株核苷酸同源性仅为88.43%。系统发育分析表明GToV/SWUN/SC与AToV具有密切的进化关系。结构分析显示HE蛋白有三种不同的结构变异，S基因有多个氨基酸突变，可能影响宿主的适应性。RT-PCR检测阳性率为35.9%(240/669)，提示GToV在四川、重庆、云南等地广泛流行。本研究增进了对环状病毒流行病学和进化的认识，为进一步研究病毒多样性提供了理论基础。

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Identification of a new goat torovirus strain: first detection and genomic analysis in China.

Using viral metagenomics, we identified a novel torovirus, GToV/SWUN/SC, in diarrheal goat fecal samples with a genome length of 28,457 nt. This strain shares 96.73-96.79% nucleotide identity with Antelope torovirus (AToV) but only 88.43% with the GToV/SZ strain. Phylogenetic analysis revealed a close evolutionary relationship between GToV/SWUN/SC and AToV. Structural analysis showed three distinct structural variations in the HE protein and multiple amino acid mutations in the S gene, which may influence host adaptation. RT-PCR detected a 35.9% (240/669) positivity rate, indicating widespread circulation of GToV in Sichuan, Chongqing, and Yunnan. This study enhances the understanding of torovirus epidemiology and evolution, providing a theoretical basis for further research on viral diversity.

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来源期刊

Irish Veterinary Journal 农林科学-兽医学

CiteScore

4.80

自引率

3.40%

发文量

审稿时长

>36 weeks

期刊介绍： Irish Veterinary Journal is an open access journal with a vision to make a substantial contribution to the dissemination of evidence-based knowledge that will promote optimal health and welfare of both domestic and wild species of animals. Irish Veterinary Journal has a clinical research focus with an emphasis on the effective management of health in both individual and populations of animals. Published studies will be relevant to both the international veterinary profession and veterinary scientists. Papers relating to veterinary education, veterinary ethics, veterinary public health, or relevant studies in the area of social science (participatory research) are also within the scope of Irish Veterinary Journal.