串联俘获离子迁移率光谱法的差分熔化电压:聚糖结构影响糖蛋白稳定性。

IF 3.1 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mengqi Chai, Christian Bleiholder, Fanny C Liu
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引用次数: 0

摘要

分析蛋白质糖型的全谱对于理解它们的功能作用至关重要。我们利用串联离子迁移率/串联质谱法开发了差分熔化电压方法,并将其应用于核糖核酸酶B糖型的研究。我们的研究结果表明,除了聚糖质量和完整的蛋白质大小外,聚糖结构还对核糖核酸酶B的稳定性起着调节作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differential melting voltage by tandem-trapped ion mobility spectrometry: glycan structure influences glycoprotein stability.

Profiling the full spectrum of protein glycoforms is critical to understanding their functional roles. We developed the differential melting voltage approach using tandem-ion mobility/tandem-mass spectrometry and applied it to study Ribonuclease B glycoforms. Our results indicate that, in addition to glycan mass and intact protein size, the glycan structure plays a role in regulating the stability of Ribonuclease B.

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来源期刊
CiteScore
6.10
自引率
0.00%
发文量
128
审稿时长
10 weeks
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