基于DNA界面的多层可转换结构的一体化生物计算纳米试剂。

IF 13.3 1区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Theranostics Pub Date : 2025-07-25 eCollection Date: 2025-01-01 DOI:10.7150/thno.113059
Vladimir R Cherkasov, Elizaveta N Mochalova, Andrey V Babenyshev, Maxim P Nikitin
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引用次数: 0

摘要

病原体渗入宿主生物的多样性突出了对同样复杂的预防机制的需求。具有分子逻辑能力的“智能”代理的发展充满希望,但它们的全部治疗潜力尚未实现。方法:基于“基于粒子分解的生物计算”技术的纳米试剂的原始概念已扩展到核酸(NAs)接口和输入。通过利用NAs的独特特性,我们设计的纳米结构可以通过由寡核苷酸输入触发的DNA链位移,在统一的纳米粒子平台上实现所有基本的单输入和双输入逻辑门。研究了纳米结构在不同输出信号检测格式下的性能,包括与纳米物体和靶向细胞的特定相互作用。结果:在这里,我们展示了基于纳米颗粒和DNA界面(“DNA转换器”)的自主治疗生物计算代理,能够在单个一体化粒子结构中执行一套功能完整的布尔逻辑门(YES, NOT, and和OR)。每个dna转换器都是通过纳米颗粒通过dna界面进行多层自组装而构建的。由特定ssDNA输入的特定组合诱导的药物分解途径决定了药物产生与治疗应用(如特异性靶向HER2/新阳性癌细胞)兼容的程序化输出的能力。结论:所开发的一体化dna纳米试剂代表了分子逻辑器件的重大进步,为智能纳米试剂的诊断和治疗应用建立了一个通用的平台。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
All-in-one Biocomputing Nanoagents with Multilayered Transformable Architecture based on DNA Interfaces.

The pathogen diversity to infiltrate the host organism highlights the demand for equally sophisticated mechanisms for their prevention. The development of "intelligent" agents with molecular logic capabilities are of great hope, but their full theranostic potential has yet to be realized. Methods: The original concept of nanoagents based on "Biocomputing based on particle disassembly" technology has been extended to nucleic acids (NAs) interfaces and inputs. By exploiting the unique properties of NAs, we designed nanostructures that can implement all basic single- and dual-input logic gates on a unified nanoparticle platform through DNA strand displacement triggered by oligonucleotide inputs. Performance of nanostructures was investigated across various output signal detection formats including specific interaction with nanosized objects and targeting cells. Results: Here, we demonstrate autonomous theranostic biocomputing agents based on nanoparticles and DNA interfaces ("DNA-transformers") capable of executing a functionally complete set of Boolean logic gates (YES, NOT, AND, and OR) within a single all-in-one particle structure. Each DNA-transformer is constructed through a multi-layered self-assembly of nanoparticles via DNA-interfaces. The route of the agent's disassembly induced by the particular combination of the specific ssDNA inputs determines the agents' ability to produce the programmed outputs compatible with theranostic applications such as specific targeting of HER2/neu-positive cancer cells. Conclusions: The developed all-in-one DNA-based nanoagents represent a significant advancement in molecular logic devices, establishing a versatile platform for smart nanoagents equally suitable for diagnostic and therapeutic applications.

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来源期刊
Theranostics
Theranostics MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
25.40
自引率
1.60%
发文量
433
审稿时长
1 months
期刊介绍: Theranostics serves as a pivotal platform for the exchange of clinical and scientific insights within the diagnostic and therapeutic molecular and nanomedicine community, along with allied professions engaged in integrating molecular imaging and therapy. As a multidisciplinary journal, Theranostics showcases innovative research articles spanning fields such as in vitro diagnostics and prognostics, in vivo molecular imaging, molecular therapeutics, image-guided therapy, biosensor technology, nanobiosensors, bioelectronics, system biology, translational medicine, point-of-care applications, and personalized medicine. Encouraging a broad spectrum of biomedical research with potential theranostic applications, the journal rigorously peer-reviews primary research, alongside publishing reviews, news, and commentary that aim to bridge the gap between the laboratory, clinic, and biotechnology industries.
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