植物激酶-12 POK2尾部是一个多功能的相互作用枢纽。

IF 3.6 3区 生物学 Q3 CELL BIOLOGY
Shu Yao Leong, Laura Muras, Benedikt S J Fischer, Sehee Jang, Anastasia Gurskaya, Mayank Chugh, Serapion Pyrpassopoulos, Hauke Drechsler, Erik Schäffer
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引用次数: 0

摘要

组织的发育和功能依赖于正确的细胞模式。在植物中,模式是由有丝分裂过程中形成的新细胞壁决定的。在胚胎前期,环状的位置线索在质膜上标志着未来的分裂平面。这些信号包括肌动蛋白12马达PHRAGMOPLAST定向肌动蛋白2 (POK2)及其平行蛋白POK1。它们对于正确排列膜质体是必不可少的,膜质体是细胞壁形成的微管支架。虽然我们对这些马达是如何对齐药膜有了基本的了解,但我们目前缺乏关于它们是如何定位和维持在质膜上的信息。本研究在体外微管和囊泡上重组了重组POK2尾部片段。我们发现POK2尾部与微管、微管相关蛋白MAP65-3以及某些阴离子脂质相互作用。我们在POK2尾部确定了一个短元素,负责所有三种交互作用。我们的数据表明,一个顺序和合作的机制,将POK2特异性地靶向未来的分裂位点。在那里,它通过与质膜的相互作用来建立细胞分裂平面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The plant kinesin-12 POK2 tail is a versatile interaction hub.

Tissue development and function rely on correct cell patterning. In plants, patterns are determined by the new cell wall formed during mitosis. Already in preprophase, ring-like, positional cues mark the future division plane on the plasma membrane in embryophytes. These cues include the kinesin-12 motors PHRAGMOPLAST ORIENTING KINESIN 2 (POK2) and its paralogue POK1. They are essential for correctly aligning the phragmoplast - a microtubule scaffold for cell wall formation. Although we have a basic understanding of how these motors align the phragmoplast, we currently lack information on how they are targeted and maintained at the plasma membrane. Here, we reconstituted recombinant POK2 tail fragments on microtubules and vesicles in vitro. We found that the POK2 tail interacted with microtubules, the microtubule-associated protein MAP65-3, as well as with certain anionic lipids. We identified a short element in the POK2 tail responsible for all three interactions. Our data suggest a sequential and cooperative mechanism that targets POK2 specifically to the future division site. There, it is robustly maintained through interactions with the plasma membrane to establish the cell division plane.

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来源期刊
Journal of cell science
Journal of cell science 生物-细胞生物学
CiteScore
7.30
自引率
2.50%
发文量
393
审稿时长
1.4 months
期刊介绍: Journal of Cell Science publishes cutting-edge science, encompassing all aspects of cell biology.
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