Tingxiu Jiang, Hong Chen, Chunjie Qin, Guoran Xie, Qiumei Huang, Shaomei Chen, Yongrong Lai
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Potential prognostic value and immune landscape of lactylation and liquid-liquid phase separation related genes in acute myeloid leukemia.
Objectives: Lactylation- and liquid-liquid phase separation-related differentially expressed genes (LLRDEGs) have been implicated in cancer. However, their role in acute myeloid leukemia (AML) remains largely unexplored.
Methods: LLRDEGs associated with AML prognosis were identified using Cox regression and LASSO analyzes. A prognostic model based on three LLRDEGs was constructed for AML, and its associated biological functions were investigated. Furthermore, we evaluated differences in the tumor immune microenvironment based on this prognostic signature.
Results: This study represents the first comprehensive analysis of the prognostic value of LLRDEGs in AML patients, identifying three LLRDEGs with prognostic significance. A prognostic risk model was constructed using these three LLRDEGs, and its prognostic value was validated in an independent external AML dataset. This model was associated with the immune microenvironment of AML. Finally, we observed that chaperonin containing TCP1 subunit 5 (CCT5) was highly expressed in AML. In vitro experiments demonstrated that inhibition of CCT5 induces HL-60 cell cycle arrest and apoptosis.
Conclusion: This research provides a theoretical basis relevant to AML treatment strategies.
期刊介绍：
Hematology is an international journal publishing original and review articles in the field of general hematology, including oncology, pathology, biology, clinical research and epidemiology. Of the fixed sections, annotations are accepted on any general or scientific field: technical annotations covering current laboratory practice in general hematology, blood transfusion and clinical trials, and current clinical practice reviews the consensus driven areas of care and management.
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