Mariane A. P. Silva, Mónica Cappetta, Tony F. Grassi, Leandro G. Braz, Mariana G. Braz
{"title":"职业接触废麻醉气体的分子影响。","authors":"Mariane A. P. Silva, Mónica Cappetta, Tony F. Grassi, Leandro G. Braz, Mariana G. Braz","doi":"10.1002/em.70029","DOIUrl":null,"url":null,"abstract":"<p>Millions of healthcare professionals are occupationally exposed to waste anesthetic gases (WAGs) worldwide; however, their effect on DNA methylation is unknown. Thus, for the first time, global DNA methylation analysis was performed in addition to the comet assay and gene expression analysis in this case–control study to reveal possible molecular alterations associated with WAG exposure. A total of 86 participants were included (43 healthcare professionals exposed to WAGs and 43 matched-controls). Mononuclear cells were isolated for the comet assay, and DNA was extracted from whole blood for global DNA methylation analysis. RNA was isolated for the gene expression of <i>NRF2</i>, <i>SIRT1</i>, and <i>HO1</i>. Increased DNA damage was observed in the exposed group (<i>p</i> = 0.02). No differences in global DNA methylation or <i>HO1</i> expression were observed (<i>p</i> > 0.05), but <i>SIRT1</i> (<i>p</i> = 0.01) and <i>NRF2</i> (<i>p</i> = 0.02) upregulation were observed in the exposed group. These findings highlight the molecular effects of WAG exposure.</p>","PeriodicalId":11791,"journal":{"name":"Environmental and Molecular Mutagenesis","volume":"66 6-7","pages":"397-402"},"PeriodicalIF":2.3000,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/em.70029","citationCount":"0","resultStr":"{\"title\":\"Molecular Impacts of Occupational Exposure to Waste Anesthetic Gases\",\"authors\":\"Mariane A. P. Silva, Mónica Cappetta, Tony F. Grassi, Leandro G. Braz, Mariana G. Braz\",\"doi\":\"10.1002/em.70029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Millions of healthcare professionals are occupationally exposed to waste anesthetic gases (WAGs) worldwide; however, their effect on DNA methylation is unknown. Thus, for the first time, global DNA methylation analysis was performed in addition to the comet assay and gene expression analysis in this case–control study to reveal possible molecular alterations associated with WAG exposure. A total of 86 participants were included (43 healthcare professionals exposed to WAGs and 43 matched-controls). Mononuclear cells were isolated for the comet assay, and DNA was extracted from whole blood for global DNA methylation analysis. RNA was isolated for the gene expression of <i>NRF2</i>, <i>SIRT1</i>, and <i>HO1</i>. Increased DNA damage was observed in the exposed group (<i>p</i> = 0.02). No differences in global DNA methylation or <i>HO1</i> expression were observed (<i>p</i> > 0.05), but <i>SIRT1</i> (<i>p</i> = 0.01) and <i>NRF2</i> (<i>p</i> = 0.02) upregulation were observed in the exposed group. These findings highlight the molecular effects of WAG exposure.</p>\",\"PeriodicalId\":11791,\"journal\":{\"name\":\"Environmental and Molecular Mutagenesis\",\"volume\":\"66 6-7\",\"pages\":\"397-402\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-08-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/em.70029\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental and Molecular Mutagenesis\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/em.70029\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental and Molecular Mutagenesis","FirstCategoryId":"93","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/em.70029","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Molecular Impacts of Occupational Exposure to Waste Anesthetic Gases
Millions of healthcare professionals are occupationally exposed to waste anesthetic gases (WAGs) worldwide; however, their effect on DNA methylation is unknown. Thus, for the first time, global DNA methylation analysis was performed in addition to the comet assay and gene expression analysis in this case–control study to reveal possible molecular alterations associated with WAG exposure. A total of 86 participants were included (43 healthcare professionals exposed to WAGs and 43 matched-controls). Mononuclear cells were isolated for the comet assay, and DNA was extracted from whole blood for global DNA methylation analysis. RNA was isolated for the gene expression of NRF2, SIRT1, and HO1. Increased DNA damage was observed in the exposed group (p = 0.02). No differences in global DNA methylation or HO1 expression were observed (p > 0.05), but SIRT1 (p = 0.01) and NRF2 (p = 0.02) upregulation were observed in the exposed group. These findings highlight the molecular effects of WAG exposure.
期刊介绍：
Environmental and Molecular Mutagenesis publishes original research manuscripts, reviews and commentaries on topics related to six general areas, with an emphasis on subject matter most suited for the readership of EMM as outlined below. The journal is intended for investigators in fields such as molecular biology, biochemistry, microbiology, genetics and epigenetics, genomics and epigenomics, cancer research, neurobiology, heritable mutation, radiation biology, toxicology, and molecular & environmental epidemiology.
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