儿童多药耐药社区相关MRSA中Tn552- ii （Tn552衍生物）到共轭pCtra质粒家族的转位

IF 4.5 2区医学 Q2 MICROBIOLOGY

Antimicrobial Agents and Chemotherapy Pub Date : 2025-10-01 Epub Date: 2025-09-03 DOI:10.1128/aac.00647-25

Tsai-Wen Wan, Masaki Shintani, Kenji K Kojima, Naohiko Moriguchi, Wei-Chun Hung, Yu-Ting Wang, Lee-Jene Teng, Tatsuo Yamamoto

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引用次数: 0

摘要

我们报道了携带tnpABC/靶基因样(tgl)/ blaza - r1 - i阵列的9045 bp转座子（Tn552-II）转位到儿童多药耐药社区相关耐甲氧西林金黄色葡萄球菌的共轭质粒（pCtra）上。Tn552-II在左侧含有一个7bp的靶位（att）；右边是一个内部i-att，显然是通过一个圆形中间体“引入”的；以及TGL基因及其邻近基因中的i-att2和i-att3。这种转位增强了氨苄西林耐药性，并促进了Tn552-II以10-3的频率传播。

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Transposition of Tn552-II (a Tn552 derivative) to the conjugative pCtra plasmid family in pediatric multidrug-resistant community-associated MRSA.

We report the transposition of a 9,045-bp transposon (Tn552-II) carrying the tnpABC/target-gene-like(tgl)/blaZ-R1-I array to a conjugative plasmid (pCtra) in pediatric multidrug-resistant community-associated methicillin-resistant Staphylococcus aureus. Tn552-II contained a 7-bp target site (att) on the left; an internal i-att on the right, apparently "brought-in" through a circular intermediate; and i-att2 and i-att3 in the tgl gene and its neighboring gene, respectively. This transposition enhanced ampicillin resistance and facilitated Tn552-II spread at a frequency of 10^-3.

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来源期刊

Antimicrobial Agents and Chemotherapy 医学-微生物学

CiteScore

10.00

自引率

8.20%

发文量

762

审稿时长

3 months

期刊介绍： Antimicrobial Agents and Chemotherapy (AAC) features interdisciplinary studies that build our understanding of the underlying mechanisms and therapeutic applications of antimicrobial and antiparasitic agents and chemotherapy.