肝门周围胆管癌中拷贝中性的杂合性缺失模式的发现具有潜在的预后价值。

IF 1.7 4区医学 Q4 ONCOLOGY

Anticancer research Pub Date : 2025-09-01 DOI:10.21873/anticanres.17734

Sutheemon Techa-Ay, Sasithorn Watcharadetwittaya, Raksawan Deenonpoe, Kitti Intuyod, Anchalee Techasen, Poramate Klanrit, Watcharin Loilome, Prakasit Sa-Ngiamwibool, Malinee Thanee

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引用次数: 0

摘要

背景/目的：肝门周围胆管癌（pCCA）是一种预后差的侵袭性胆管癌（切除后5年生存率为10-40%），主要见于泰国，预后模型有限。拷贝中性杂合性缺失（cnLOH）在各种癌症中影响其进展，导致较差的总生存期。本研究旨在探讨个体pCCA患者的cnLOH模式，以建立预后生物标志物的预测模型。材料和方法：从14个冷冻组织中提取DNA，用染色体微阵列（CMA）分析cnLOH。结果：cnLOH分析显示，每个样本中位数为14个事件。在染色体1q上观察到最常见的cnLOH，其次是染色体2、3p、5q、6q、7q、11q和12q。重要的是，我们发现了几个特定的cnLOH区域（2p23.2、2p23.3和6p11.1），这些区域的改变与总生存期的降低显著相关(结论：这些结果提供了cnLOH事件可能有助于pCCA患者的疾病进展和影响总生存期的证据，表明cnLOH事件在这种恶性肿瘤的潜在分子机制中具有潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Discovery of a Copy-neutral Loss of Heterozygosity Pattern in Perihilar Cholangiocarcinoma With Potential Prognostic Value.

Background/aim: Perihilar cholangiocarcinoma (pCCA) is an aggressive bile duct cancer with a poor prognosis (10-40% 5-year survival after resection), that is mostly found in Thailand and has limited prognostic models. Copy neutral loss of heterozygosity (cnLOH) in various cancers affects their progression leading to poorer overall survival. This study aimed to explore cnLOH patterns in individual pCCA patients in order to develop a predictive model for prognostic biomarkers.

Materials and methods: DNA was extracted from 14 frozen tissues and chromosome microarray (CMA) was performed to analyze cnLOH.

Results: Analysis of cnLOH revealed a median of 14 events per sample. The most frequent cnLOH was observed on chromosome 1q, followed by high occurrences on chromosomes 2, 3p, 5q, 6q, 7q, 11q, and 12q. Importantly, we identified several specific cnLOH regions (2p23.2, 2p23.3, and 6p11.1) where alterations were significantly associated with decreased overall survival (p<0.05).

Conclusion: These results provide evidence that cnLOH events may contribute to disease progression and influence overall survival in patients with pCCA, indicating a potential role in the underlying molecular mechanisms of this malignancy.

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来源期刊

Anticancer research 医学-肿瘤学

CiteScore

3.70

自引率

10.00%

发文量

566

审稿时长

2 months

期刊介绍： ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.