{"title":"磷酸化yes相关蛋白（YAP） （Ser 127）在口腔鳞状细胞癌中的表达","authors":"Yusuke Amano, Daisuke Matsubara, Atsushi Kihara, Taichiro Yoshimoto, Noriyoshi Fukushima, Hiroshi Nishino, Yoshiyuki Mori, Kentaro Inamura, Toshiro Niki","doi":"10.21873/anticanres.17743","DOIUrl":null,"url":null,"abstract":"<p><strong>Background/aim: </strong>The Hippo signaling pathway comprises mammalian sterile 20-like kinase 1/2, large tumor suppressor 1/2, and Yes-associated protein 1 (YAP1). This study investigated phosphorylated YAP (pYAP, Ser 127) protein expression in oral squamous cell carcinoma (OSCC).</p><p><strong>Patients and methods: </strong>Tissues from patients with oral epithelial dysplasia (OED, n=7), carcinoma <i>in situ</i> (CIS, n=14), and OSCC (n=109) were analyzed.</p><p><strong>Results: </strong>Cytoplasmic expression of pYAP was low in OED, CIS, and OSCC tissues. The expression of pYAP was correlated with differentiation, and the expression of low levels of YAP was significantly more common in well-differentiated to moderately differentiated OSCC than in poorly differentiated OSCC. High pYAP expression correlates with characteristics of epithelial-to-mesenchymal transition (EMT), <i>e.g.</i>, loss of E-cadherin and increased expression of vimentin and laminin 5 (<i>p</i><0.0001). Additionally, the protein arginine methyltransferase 1, a positive modulatory factor of YAP activity, was found to be correlated with elevated levels of pYAP expression (<i>p</i><0.0007).</p><p><strong>Conclusion: </strong>The presence of elevated pYAP expression may serve as a prognostic indicator of an aggressive OSCC with EMT during the invasive stage.</p>","PeriodicalId":8072,"journal":{"name":"Anticancer research","volume":"45 9","pages":"3835-3845"},"PeriodicalIF":1.7000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Significance of Phosphorylated Yes-associated Protein (YAP) (Ser 127) Expression in Oral Squamous Cell Carcinoma.\",\"authors\":\"Yusuke Amano, Daisuke Matsubara, Atsushi Kihara, Taichiro Yoshimoto, Noriyoshi Fukushima, Hiroshi Nishino, Yoshiyuki Mori, Kentaro Inamura, Toshiro Niki\",\"doi\":\"10.21873/anticanres.17743\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background/aim: </strong>The Hippo signaling pathway comprises mammalian sterile 20-like kinase 1/2, large tumor suppressor 1/2, and Yes-associated protein 1 (YAP1). This study investigated phosphorylated YAP (pYAP, Ser 127) protein expression in oral squamous cell carcinoma (OSCC).</p><p><strong>Patients and methods: </strong>Tissues from patients with oral epithelial dysplasia (OED, n=7), carcinoma <i>in situ</i> (CIS, n=14), and OSCC (n=109) were analyzed.</p><p><strong>Results: </strong>Cytoplasmic expression of pYAP was low in OED, CIS, and OSCC tissues. The expression of pYAP was correlated with differentiation, and the expression of low levels of YAP was significantly more common in well-differentiated to moderately differentiated OSCC than in poorly differentiated OSCC. High pYAP expression correlates with characteristics of epithelial-to-mesenchymal transition (EMT), <i>e.g.</i>, loss of E-cadherin and increased expression of vimentin and laminin 5 (<i>p</i><0.0001). Additionally, the protein arginine methyltransferase 1, a positive modulatory factor of YAP activity, was found to be correlated with elevated levels of pYAP expression (<i>p</i><0.0007).</p><p><strong>Conclusion: </strong>The presence of elevated pYAP expression may serve as a prognostic indicator of an aggressive OSCC with EMT during the invasive stage.</p>\",\"PeriodicalId\":8072,\"journal\":{\"name\":\"Anticancer research\",\"volume\":\"45 9\",\"pages\":\"3835-3845\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anticancer research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.21873/anticanres.17743\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ONCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anticancer research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.21873/anticanres.17743","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景/目的：Hippo信号通路包括哺乳动物不育20样激酶1/2、大肿瘤抑制因子1/2和yes相关蛋白1 （YAP1）。本研究探讨了磷酸化YAP （pYAP, Ser 127）蛋白在口腔鳞状细胞癌（OSCC）中的表达。患者和方法：对口腔上皮发育不良（OED, n=7）、原位癌（CIS, n=14）和OSCC （n=109）患者的组织进行分析。结果：pYAP在OED、CIS和OSCC组织中表达较低。pYAP的表达与分化相关，低水平的YAP表达在高分化至中分化的OSCC中比在低分化的OSCC中更为常见。pYAP的高表达与上皮-间质转化（EMT）的特征相关，例如E-cadherin的缺失以及vimentin和层粘连蛋白5的表达增加(pp结论：pYAP表达升高可能是侵袭性OSCC合并EMT侵袭期的预后指标。
The Significance of Phosphorylated Yes-associated Protein (YAP) (Ser 127) Expression in Oral Squamous Cell Carcinoma.
Background/aim: The Hippo signaling pathway comprises mammalian sterile 20-like kinase 1/2, large tumor suppressor 1/2, and Yes-associated protein 1 (YAP1). This study investigated phosphorylated YAP (pYAP, Ser 127) protein expression in oral squamous cell carcinoma (OSCC).
Patients and methods: Tissues from patients with oral epithelial dysplasia (OED, n=7), carcinoma in situ (CIS, n=14), and OSCC (n=109) were analyzed.
Results: Cytoplasmic expression of pYAP was low in OED, CIS, and OSCC tissues. The expression of pYAP was correlated with differentiation, and the expression of low levels of YAP was significantly more common in well-differentiated to moderately differentiated OSCC than in poorly differentiated OSCC. High pYAP expression correlates with characteristics of epithelial-to-mesenchymal transition (EMT), e.g., loss of E-cadherin and increased expression of vimentin and laminin 5 (p<0.0001). Additionally, the protein arginine methyltransferase 1, a positive modulatory factor of YAP activity, was found to be correlated with elevated levels of pYAP expression (p<0.0007).
Conclusion: The presence of elevated pYAP expression may serve as a prognostic indicator of an aggressive OSCC with EMT during the invasive stage.
期刊介绍：
ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed.
ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies).
Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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