用多级线性离子阱-高分辨质谱法鉴定含有新型脂系的黄体微球菌脂质体。

IF 2.7 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Brian A. Kleiboeker,  and , Fong-Fu Hsu*, 
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引用次数: 0

摘要

黄体微球菌(M. luteus)是一种普遍存在的,长期存在的革兰氏阳性细菌,具有独特的黄色或金色。由于其基因组小,易于培养,因此是实验室研究的模式生物。然而,对其组成脂质结构的了解有限,其整个脂质谱仍不清楚。在这里,我们应用线性离子陷阱(LIT)多级质谱(MSn)对天然脂质提取物进行了高分辨率的结构表征,并对酸水解产物进行了GC/MS分析,以揭示整个脂质组的结构细节,包括主要的磷脂酰甘油(PG)、心磷脂(CL)、磷脂酰肌醇(PI)、二甘油酯二酰基甘油(DMDG)和次要的二酰基甘油(DAG)脂质家族。重要的是,我们还发现了两个额外的脂质家族,新的磷脂酰1,3-丙二醇和已知的聚戊烯酰1-磷酸,这在以前没有报道过。我们还发现了由支链15:0-脂肪酸取代基(>90%支链15:0-FA,前15:0- iso-15:0丰度比为4/1)所简化的独特脂质组,这与黄曲霉的小基因组一致。此外,应用LIT - MSn质谱法揭示了DMDG特有的甘油残留物内部损失的破碎途径,从而导致其结构表征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Characterization of Micrococcus luteus Lipidome Containing Novel Lipid Families by Multiple Stage Linear Ion-Trap with High Resolution Mass Spectrometry

Characterization of Micrococcus luteus Lipidome Containing Novel Lipid Families by Multiple Stage Linear Ion-Trap with High Resolution Mass Spectrometry

Micrococcus luteus (M. luteus) is a ubiquitous, long-existing Gram-positive bacterium with a distinctive yellow or golden color. It is a model organism for laboratory studies due to its small genome and ease of cultivation. However, only limited knowledge about its constituent lipid structure is known, and its entire lipid profile remains unclear. Here, we applied linear ion trap (LIT) multiple-stage mass spectrometry (MSn) with high resolution for structural characterization of the native lipid extract, along with GC/MS analysis of the acid hydrolysate to reveal the structural details of the entire lipidome, which includes the major phosphatidylglycerol (PG), cardiolipin (CL), phosphatidylinositol (PI), dimannosyl diacylglycerol (DMDG), and minor diacylglycerol (DAG) lipid families. Importantly, we also found two extra lipid families, the new phosphatidyl 1,3-propanediol and the known polyprenyl 1-phosphosate that was not previously reported for M. luteus. We also revealed the unique lipidome simplified by the dominance of branched 15:0-fatty acid substituents (>90% branched 15:0-FA with anteiso-15:0 to iso-15:0 abundance ratio of 4/1), which is in line with the small genome of M. luteus. In addition, the applied LIT MSn mass spectrometry revealed a fragmentation pathway that undergoes internal loss of a glycerol residue specific to DMDG, leading to its structural characterization.

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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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