{"title":"用多级线性离子阱-高分辨质谱法鉴定含有新型脂系的黄体微球菌脂质体。","authors":"Brian A. Kleiboeker, and , Fong-Fu Hsu*, ","doi":"10.1021/jasms.5c00133","DOIUrl":null,"url":null,"abstract":"<p ><i>Micrococcus luteus</i> (<i>M. luteus</i>) is a ubiquitous, long-existing Gram-positive bacterium with a distinctive yellow or golden color. It is a model organism for laboratory studies due to its small genome and ease of cultivation. However, only limited knowledge about its constituent lipid structure is known, and its entire lipid profile remains unclear. Here, we applied linear ion trap (LIT) multiple-stage mass spectrometry (MS<i><sup>n</sup></i>) with high resolution for structural characterization of the native lipid extract, along with GC/MS analysis of the acid hydrolysate to reveal the structural details of the entire lipidome, which includes the major phosphatidylglycerol (PG), cardiolipin (CL), phosphatidylinositol (PI), dimannosyl diacylglycerol (DMDG), and minor diacylglycerol (DAG) lipid families. Importantly, we also found two extra lipid families, the new phosphatidyl 1,3-propanediol and the known polyprenyl 1-phosphosate that was not previously reported for <i>M. luteus</i>. We also revealed the unique lipidome simplified by the dominance of branched 15:0-fatty acid substituents (>90% branched 15:0-FA with anteiso-15:0 to iso-15:0 abundance ratio of 4/1), which is in line with the small genome of <i>M. luteus</i>. In addition, the applied LIT MS<sup><i>n</i></sup> mass spectrometry revealed a fragmentation pathway that undergoes internal loss of a glycerol residue specific to DMDG, leading to its structural characterization.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 10","pages":"2117–2125"},"PeriodicalIF":2.7000,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of Micrococcus luteus Lipidome Containing Novel Lipid Families by Multiple Stage Linear Ion-Trap with High Resolution Mass Spectrometry\",\"authors\":\"Brian A. Kleiboeker, and , Fong-Fu Hsu*, \",\"doi\":\"10.1021/jasms.5c00133\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p ><i>Micrococcus luteus</i> (<i>M. luteus</i>) is a ubiquitous, long-existing Gram-positive bacterium with a distinctive yellow or golden color. It is a model organism for laboratory studies due to its small genome and ease of cultivation. However, only limited knowledge about its constituent lipid structure is known, and its entire lipid profile remains unclear. Here, we applied linear ion trap (LIT) multiple-stage mass spectrometry (MS<i><sup>n</sup></i>) with high resolution for structural characterization of the native lipid extract, along with GC/MS analysis of the acid hydrolysate to reveal the structural details of the entire lipidome, which includes the major phosphatidylglycerol (PG), cardiolipin (CL), phosphatidylinositol (PI), dimannosyl diacylglycerol (DMDG), and minor diacylglycerol (DAG) lipid families. Importantly, we also found two extra lipid families, the new phosphatidyl 1,3-propanediol and the known polyprenyl 1-phosphosate that was not previously reported for <i>M. luteus</i>. We also revealed the unique lipidome simplified by the dominance of branched 15:0-fatty acid substituents (>90% branched 15:0-FA with anteiso-15:0 to iso-15:0 abundance ratio of 4/1), which is in line with the small genome of <i>M. luteus</i>. In addition, the applied LIT MS<sup><i>n</i></sup> mass spectrometry revealed a fragmentation pathway that undergoes internal loss of a glycerol residue specific to DMDG, leading to its structural characterization.</p>\",\"PeriodicalId\":672,\"journal\":{\"name\":\"Journal of the American Society for Mass Spectrometry\",\"volume\":\"36 10\",\"pages\":\"2117–2125\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-09-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the American Society for Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/jasms.5c00133\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Society for Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/jasms.5c00133","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Characterization of Micrococcus luteus Lipidome Containing Novel Lipid Families by Multiple Stage Linear Ion-Trap with High Resolution Mass Spectrometry
Micrococcus luteus (M. luteus) is a ubiquitous, long-existing Gram-positive bacterium with a distinctive yellow or golden color. It is a model organism for laboratory studies due to its small genome and ease of cultivation. However, only limited knowledge about its constituent lipid structure is known, and its entire lipid profile remains unclear. Here, we applied linear ion trap (LIT) multiple-stage mass spectrometry (MSn) with high resolution for structural characterization of the native lipid extract, along with GC/MS analysis of the acid hydrolysate to reveal the structural details of the entire lipidome, which includes the major phosphatidylglycerol (PG), cardiolipin (CL), phosphatidylinositol (PI), dimannosyl diacylglycerol (DMDG), and minor diacylglycerol (DAG) lipid families. Importantly, we also found two extra lipid families, the new phosphatidyl 1,3-propanediol and the known polyprenyl 1-phosphosate that was not previously reported for M. luteus. We also revealed the unique lipidome simplified by the dominance of branched 15:0-fatty acid substituents (>90% branched 15:0-FA with anteiso-15:0 to iso-15:0 abundance ratio of 4/1), which is in line with the small genome of M. luteus. In addition, the applied LIT MSn mass spectrometry revealed a fragmentation pathway that undergoes internal loss of a glycerol residue specific to DMDG, leading to its structural characterization.
期刊介绍:
The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role.
Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives