操纵酿酒酵母麦角甾醇生物合成途径合成甾醇及其相关天然产物的研究进展

IF 3.9 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

ACS Synthetic Biology Pub Date : 2025-08-27 DOI:10.1021/acssynbio.5c00438

Yinmiao Wang, Yiqi Jiang* and Jianping Lin*,

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引用次数: 0

摘要

甾醇是细胞膜的重要结构成分，具有重要的生物学功能，是农业和制药工业的关键中间体。合成生物学的最新进展已经确立了酿酒酵母作为甾醇生物合成的强大微生物基础。通过对异源途径的战略性整合和代谢通量的系统性增强，该真核平台能够重新合成多种高价值类固醇。本文系统地综述了酿酒酵母代谢工程在甾醇生物合成和过量生产方面的最新进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Progress in the Synthesis of Sterols and Related Natural Products by Manipulating the Ergosterol Biosynthetic Pathway in Saccharomyces cerevisiae

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Progress in the Synthesis of Sterols and Related Natural Products by Manipulating the Ergosterol Biosynthetic Pathway in Saccharomyces cerevisiae

Sterols, crucial structural components of cellular membranes, exhibit essential biological functions and serve as key intermediates in the agricultural and pharmaceutical industries. Recent advances in synthetic biology have established Saccharomyces cerevisiae as a robust microbial chassis for sterol biosynthesis. Through strategic integration of heterologous pathways and systematic enhancement of metabolic flux, this eukaryotic platform has enabled de novo synthesis of diverse high-value steroids. This review systematically examines recent progress in metabolic engineering of S. cerevisiae for sterol biosynthesis and overproduction.

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来源期刊

ACS Synthetic Biology 生物-

CiteScore

8.00

自引率

10.60%

发文量

380

审稿时长

6-12 weeks

期刊介绍： The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.