Huiqian Zhang , Xiuwei Shen , Jiayi He , Qingyuan Wang , Yunbing Tang , Peipei Pan , Ren-Shan Ge , Xiaoheng Li
{"title":"真菌毒素对胎盘功能的影响:重点关注环吡唑酸、脱氧雪腐镰刀菌醇、展霉素和玉米赤霉烯酮的代谢物","authors":"Huiqian Zhang , Xiuwei Shen , Jiayi He , Qingyuan Wang , Yunbing Tang , Peipei Pan , Ren-Shan Ge , Xiaoheng Li","doi":"10.1016/j.cbi.2025.111718","DOIUrl":null,"url":null,"abstract":"<div><div>Mycotoxins are secondary metabolites produced by fungi and pose significant risks to human and animal health. This study aimed to determine inhibitory potency of 7 mycotoxins and metabolites (cyclopiazonic acid, deoxynivalenol, patulin, zearalenone, α-zearalenol, β-zearalenol, and β-zearalanol) against human placental 3β-hydroxysteroid dehydrogenase 1 (h3β-HSD1) and rat homolog r3β-HSD4, as well as their effects on progesterone output in human JAr cells and perform mechanism and structure-activity relationship analysis. In placentas, h3β-HSD1 is responsible for pregnenolone-to-progesterone conversion. Cyclopiazonic acid, deoxynivalenol, patulin, α-zearalenol, β-zearalenol, and β-zearalanol significantly inhibited h3β-HSD1 activity at 100 μM, while zearalenone did not. Their IC<sub>50</sub> values ranged from 8.25 μM to 30.50 μM (patulin), and Ki values from 9.04 μM to 31.18 μM, acting as mixed inhibitors. These mycotoxins also inhibited progesterone output by JAr cells at ≥10 μM. For r3β-HSD4, similar inhibitions were observed. IC<sub>50</sub> values ranged from 10.55 μM to 42.83 μM. Docking analysis of mycotoxins with h3β-HSD1 and r3β-HSD4 showed interactions like cyclopiazonic acid binding to NAD<sup>+</sup>-binding site. Structure-activity relation analysis revealed correlations between molecular characteristics (hydrophilicity, density) and IC<sub>50</sub> values. 3D-QSAR analysis developed ten pharmacophore models, and Hypo1 was selected as the best model, which was validated by testing compounds like cyclopiazonic and patulin.</div></div>","PeriodicalId":274,"journal":{"name":"Chemico-Biological Interactions","volume":"421 ","pages":"Article 111718"},"PeriodicalIF":5.4000,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of mycotoxins on placental function: Focus on cyclopiazonic acid, deoxynivalenol, patulin, and zearalenone's metabolites\",\"authors\":\"Huiqian Zhang , Xiuwei Shen , Jiayi He , Qingyuan Wang , Yunbing Tang , Peipei Pan , Ren-Shan Ge , Xiaoheng Li\",\"doi\":\"10.1016/j.cbi.2025.111718\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Mycotoxins are secondary metabolites produced by fungi and pose significant risks to human and animal health. This study aimed to determine inhibitory potency of 7 mycotoxins and metabolites (cyclopiazonic acid, deoxynivalenol, patulin, zearalenone, α-zearalenol, β-zearalenol, and β-zearalanol) against human placental 3β-hydroxysteroid dehydrogenase 1 (h3β-HSD1) and rat homolog r3β-HSD4, as well as their effects on progesterone output in human JAr cells and perform mechanism and structure-activity relationship analysis. In placentas, h3β-HSD1 is responsible for pregnenolone-to-progesterone conversion. Cyclopiazonic acid, deoxynivalenol, patulin, α-zearalenol, β-zearalenol, and β-zearalanol significantly inhibited h3β-HSD1 activity at 100 μM, while zearalenone did not. Their IC<sub>50</sub> values ranged from 8.25 μM to 30.50 μM (patulin), and Ki values from 9.04 μM to 31.18 μM, acting as mixed inhibitors. These mycotoxins also inhibited progesterone output by JAr cells at ≥10 μM. For r3β-HSD4, similar inhibitions were observed. IC<sub>50</sub> values ranged from 10.55 μM to 42.83 μM. Docking analysis of mycotoxins with h3β-HSD1 and r3β-HSD4 showed interactions like cyclopiazonic acid binding to NAD<sup>+</sup>-binding site. Structure-activity relation analysis revealed correlations between molecular characteristics (hydrophilicity, density) and IC<sub>50</sub> values. 3D-QSAR analysis developed ten pharmacophore models, and Hypo1 was selected as the best model, which was validated by testing compounds like cyclopiazonic and patulin.</div></div>\",\"PeriodicalId\":274,\"journal\":{\"name\":\"Chemico-Biological Interactions\",\"volume\":\"421 \",\"pages\":\"Article 111718\"},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2025-08-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemico-Biological Interactions\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0009279725003485\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemico-Biological Interactions","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0009279725003485","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Impact of mycotoxins on placental function: Focus on cyclopiazonic acid, deoxynivalenol, patulin, and zearalenone's metabolites
Mycotoxins are secondary metabolites produced by fungi and pose significant risks to human and animal health. This study aimed to determine inhibitory potency of 7 mycotoxins and metabolites (cyclopiazonic acid, deoxynivalenol, patulin, zearalenone, α-zearalenol, β-zearalenol, and β-zearalanol) against human placental 3β-hydroxysteroid dehydrogenase 1 (h3β-HSD1) and rat homolog r3β-HSD4, as well as their effects on progesterone output in human JAr cells and perform mechanism and structure-activity relationship analysis. In placentas, h3β-HSD1 is responsible for pregnenolone-to-progesterone conversion. Cyclopiazonic acid, deoxynivalenol, patulin, α-zearalenol, β-zearalenol, and β-zearalanol significantly inhibited h3β-HSD1 activity at 100 μM, while zearalenone did not. Their IC50 values ranged from 8.25 μM to 30.50 μM (patulin), and Ki values from 9.04 μM to 31.18 μM, acting as mixed inhibitors. These mycotoxins also inhibited progesterone output by JAr cells at ≥10 μM. For r3β-HSD4, similar inhibitions were observed. IC50 values ranged from 10.55 μM to 42.83 μM. Docking analysis of mycotoxins with h3β-HSD1 and r3β-HSD4 showed interactions like cyclopiazonic acid binding to NAD+-binding site. Structure-activity relation analysis revealed correlations between molecular characteristics (hydrophilicity, density) and IC50 values. 3D-QSAR analysis developed ten pharmacophore models, and Hypo1 was selected as the best model, which was validated by testing compounds like cyclopiazonic and patulin.
期刊介绍:
Chemico-Biological Interactions publishes research reports and review articles that examine the molecular, cellular, and/or biochemical basis of toxicologically relevant outcomes. Special emphasis is placed on toxicological mechanisms associated with interactions between chemicals and biological systems. Outcomes may include all traditional endpoints caused by synthetic or naturally occurring chemicals, both in vivo and in vitro. Endpoints of interest include, but are not limited to carcinogenesis, mutagenesis, respiratory toxicology, neurotoxicology, reproductive and developmental toxicology, and immunotoxicology.