Hanneda A. Fomukong, Idowu A. Aimola, Abubakar Sani, Mamman Mohammed, Halima Bello-Manga, Gloria Chechet, Joy U. Ameloko, Reuben S. Baba, Abduljabar Musa, Pecular Nwanyibunwa Okoro
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{"title":"全血CD71+网织红细胞的优化富集用于单细胞RNA测序","authors":"Hanneda A. Fomukong, Idowu A. Aimola, Abubakar Sani, Mamman Mohammed, Halima Bello-Manga, Gloria Chechet, Joy U. Ameloko, Reuben S. Baba, Abduljabar Musa, Pecular Nwanyibunwa Okoro","doi":"10.1002/cpz1.70203","DOIUrl":null,"url":null,"abstract":"<p>Reticulocytes are a group of immature red blood cells (RBCs) produced in the bone marrow and released into the bloodstream where they mature into RBCs within 1 to 2 days. They play a crucial role in assessing bone health and many hematological diseases. Isolating viable reticulocytes for use in single-cell transcriptomics has been a challenge due to their similarity to mature RBCs. Their low concentration and continuous maturation to RBCs with time also poses a great challenge. In this article, we optimized a method for enrichment of CD71<sup>+</sup> reticulocytes from whole blood, with a high yield and viability for downstream studies. © 2025 Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Blood tissue preparation and reticulocyte enrichment</p><p><b>Basic Protocol 2</b>: Visualization and determination of cell viability</p><p><b>Alternate Protocol</b>: Counting cells using a hemocytometer</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"5 9","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optimized Enrichment of CD71+ Reticulocytes From Whole Blood for Single-Cell RNA Sequencing\",\"authors\":\"Hanneda A. Fomukong, Idowu A. Aimola, Abubakar Sani, Mamman Mohammed, Halima Bello-Manga, Gloria Chechet, Joy U. Ameloko, Reuben S. Baba, Abduljabar Musa, Pecular Nwanyibunwa Okoro\",\"doi\":\"10.1002/cpz1.70203\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Reticulocytes are a group of immature red blood cells (RBCs) produced in the bone marrow and released into the bloodstream where they mature into RBCs within 1 to 2 days. They play a crucial role in assessing bone health and many hematological diseases. Isolating viable reticulocytes for use in single-cell transcriptomics has been a challenge due to their similarity to mature RBCs. Their low concentration and continuous maturation to RBCs with time also poses a great challenge. In this article, we optimized a method for enrichment of CD71<sup>+</sup> reticulocytes from whole blood, with a high yield and viability for downstream studies. © 2025 Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Blood tissue preparation and reticulocyte enrichment</p><p><b>Basic Protocol 2</b>: Visualization and determination of cell viability</p><p><b>Alternate Protocol</b>: Counting cells using a hemocytometer</p>\",\"PeriodicalId\":93970,\"journal\":{\"name\":\"Current protocols\",\"volume\":\"5 9\",\"pages\":\"\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2025-09-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current protocols\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.70203\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols","FirstCategoryId":"1085","ListUrlMain":"https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.70203","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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