灵长类动物羊膜-外胚层边界的羊膜和原始生殖细胞祖细胞cldn10驱动的谱系决定

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nikola Sekulovski, Amber E. Carleton, Anusha Rengarajan, Chien-Wei Lin, Maliha Kabir, Lauren N. Juga, Allison E. Whorton, Lauren E. Elberfeld, Jenna C. Wettstein, Jenna K. Schmidt, Thaddeus G. Golos, Kenichiro Taniguchi
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引用次数: 0

摘要

越来越多来自灵长类胚胎和体外系统的证据支持羊膜和原始生殖细胞(PGC)谱系进展细胞具有共同前体的观点。为了全面了解这一关键但知之甚少的前体及其后代,我们在单细胞水平上研究了发育中的人类多能干细胞衍生的羊膜和PGC形成模型的转录组进化。这一分析揭示了几个持续的羊水命运进展状态与国家特异性标记。此外,还发现了一种祖细胞样细胞,它具有羊膜和pgc样细胞系的双电位特征,并被CLDN10标记。引人注目的是,我们发现CLDN10的表达仅限于羊膜-外胚层边界区域在我们的人类胚胎植入后羊膜囊模型以及在胃原膜周围食蟹猴胚胎;此外,该边界区域具有羊膜和PGC祖细胞样转录特征。此外,我们的功能缺失分析显示,CLDN10促进羊膜样,但抑制pgc样命运。总之,基于本研究的单细胞转录组资源,我们在灵长类动物的羊膜-外胚层边界处鉴定了CLDN10+羊膜和PGC祖细胞样群体,并为人类的羊膜-外胚层边界处羊膜和PGC的形成提供了额外的分子线索。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CLDN10-driven lineage decision in an amnion and primordial germ cell progenitor at the amnion-epiblast boundary in primates
A growing body of evidence from primate embryos as well as in vitro systems supports the notion that amnion and primordial germ cell (PGC) lineage progressing cells share a common precursor. To gain comprehensive transcriptomic insights into this critical but poorly understood precursor and its progeny, we examine the evolving transcriptome of a developing human pluripotent stem cell-derived model of amnion and PGC formation at the single cell level. This analysis reveals several continuous amniotic fate progressing states with state-specific markers. Additionally, a progenitor-like cell, that displays bi-potential characteristics for amnion and PGC-like cell lineages and is marked by CLDN10, is identified. Strikingly, we find that expression of CLDN10 is restricted to the amnion-epiblast boundary region in our human post-implantation amniotic sac model as well as in peri-gastrula cynomolgus macaque embryos; moreover, this boundary region presents amnion and PGC progenitor-like transcriptional characteristics. Furthermore, our loss of function analysis shows that CLDN10 promotes amniotic but suppresses PGC-like fate. Overall, based on the single cell transcriptomic resource in this study, we identify a CLDN10+ amnion and PGC progenitor-like population at the amnion-epiblast boundary of the primate peri-gastrula, and present additional molecular clues as to how amnion and PGC may be formed at the amnion-epiblast boundary in human peri-gastrula. 
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来源期刊
Genome Biology
Genome Biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
21.00
自引率
3.30%
发文量
241
审稿时长
2 months
期刊介绍: Genome Biology stands as a premier platform for exceptional research across all domains of biology and biomedicine, explored through a genomic and post-genomic lens. With an impressive impact factor of 12.3 (2022),* the journal secures its position as the 3rd-ranked research journal in the Genetics and Heredity category and the 2nd-ranked research journal in the Biotechnology and Applied Microbiology category by Thomson Reuters. Notably, Genome Biology holds the distinction of being the highest-ranked open-access journal in this category. Our dedicated team of highly trained in-house Editors collaborates closely with our esteemed Editorial Board of international experts, ensuring the journal remains on the forefront of scientific advances and community standards. Regular engagement with researchers at conferences and institute visits underscores our commitment to staying abreast of the latest developments in the field.
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